Obesity And Proteomics Research In Non-obese Women Visceral Adipose Tissue Differences

Once considered a problem only in high income countries, overweight and obesity are now dramatically on the rise in low-and middle-income countries, and are becoming hazards to human health and society. They are major risk factors for a number of chronic diseases, including diabetes, cardiovascular diseases, and dyslipidemia and so on. Adipose tissue is now considered not only an energy storage organ but also an important endocrine organ. In this project, visceral adipose tissue from obesity as well as women of normal BMI was collected. Total protein of the visceral adipose tissue was extracted and selected homogeneous samples for1DLC-MS/MS by SDS-PAGE. The results of the tandem mass spectrometry were validated by MASCOT software, and the peptide false positive rate was estimated using the reverse database searching method. Protein database information is downloaded from the European Bioinformatics Institute website (http://www.ebi.ac.uk/IPI/). A total of1445peptides and proteins were identified from the control group, with theoretical MW distribution in the4.066kD-629.213kD, and theoretical pI distribution in the3.94-11.89.1288kinds of peptides and proteins were identified from the obese group, theoretical MW distribution in the3.268kD-3832.803kD, theoretical pI distribution in the3.94-12.18. A total of54peptides and proteins displayed a2-fold up-or down-regulation between the two groups. Of which,51peptides and proteins have been confirmed at the protein level and the remaining3was confirmed at the transcriptional level.34peptides and proteins down regulated in the obese group, including6unique proteins for the control group. The other20peptides and proteins up regulated in the obese, of which4are unique to the obese group.23proteins play important roles in lipid metabolism, energy metabolism, protein degradation and synthesis and other metabolic pathways. Further investigation of these peptides and proteins may help explain the relationship between obesity and relative disorders such as type2diabetes and dyslipidemia. Applying the methods in this project to further investigate the proteomics of visceral and abdominal subcutaneous fat tissue may draw a more comprehensive picture of the human adipose tissue.