Research On The Decomposed Recipe Of "Bitongling" And The Effects Of Optimizing "Bitongling" On CIA Jimpy Mice

Background and Objective: Rheumatoid arthritis （RA） is a kind of multi-systematicallyinflammatory autoimmune disease, mainly relating to ambient joints. The reason of thedisease is uncertain. It may be concerned with infection and inheritance and its pathologicchange is chronic Inflammation of joints,cell infiltration, information of panes and theerosion of cartilage and bones, which leads to the destruction and dysfunction of joints’structure. The treatment of RA aims at relief of pain, prevention of disability, maintenanceon the joints function and improvement the living standard of patients. WHO has dividedthe drugs to treat RA into improving symptoms and controlling the development ofdisease. It is a golden rule for clinic treatment. However, due to its uncertain pathogeny,there is no specific and pertinent drug. But the treatment of traditional Chinese medicine ismainly on determination of treatment based in pathogenesis obtained throughdifferentiation of symptoms and signs, and through multi-layer、multi-loop、multi-methodand multi-target way, it has great advantage to anti-inflammatory、analgesia、regulatingimmune function and prevent the development of disease. The research topic lies on"Bitongling"-the experienced prescription for RA of national famous Professor Wang Lvqiuand mentor Professor Wang Yue. To Resarch on the decomposed recipe through orthogonaldesign of T value, We research on the effects of optimizing "Bitongling"on RA, andconfirm the rationality of"Bitongling". To simplify and optimize the prescription. discussthe mechanism so as to provide experimental and theoretical basis RA’s therapeutics.Methods: There are three parts as follows: （1） object research on Kunming Jimpymice, introduction anti-inflammatory and analgesia as major indicatrix, through orthogonaldesign of T value, to analyze the interaction of"Bitongling" remedy adjuvans. （2） objectresearch on KunmingJ impy mice, introduction anti-inflammatory and analgesia as majorindicatrix, through orthogonal design of T value, to analyze the dosage of"Bitongling". （3）Using typâ…¡e collagen protein induced arthritis Jimpy mic （CIA）, laying blank andmasculine medicine control group, The effects of Bitongling’s high and middle and lowdosage are investigated on the point of view of cell mediated immunity with the response ofRA.Results: （1） Through orthogonal design of T value, to analyze the interaction of"Bitongling" remedy adjuvans, we can find Ephedra herb and common monkshood mother root are the principal agent, which are good at the anti-inflammatory and analgesia;szechwan lovage rhizome and scolopendra and peach seed are rivalry to the principalagent, to be removed; C and D and E and F are the remedy adjuvans. （2） to analyze theinteraction of "Bitongling" remedy adjuvans, we can find Ephedra herb and commonmonkshood mother root and orientvine stem are good at the anti-inflammatory andanalgesia, Their dose-effect relationship is significant; With the except of Ephedra herb,the rest of Bitongling do more effect during the increasing dosage. （3）The high dosage andthe middle dosage of Bitongling’s optimized prescription, and ciclosporin and the originBitongling all can obviously inhibit the arthrocele response of CIA Jimpy mice, decreaselevels of serum IL-1βandTNF-αand anti-câ…¡antibody in rats. inhibit activatedlymphocyte’s transference and release NO and IFN-γtransfusion, among the total,ciclosporin is the best, high dose and the original Bitongling are the second.Conclusion: （1） The optimal Chinese crude drug of"Bitongling" optimized prescription ismade up of A, C, D, E and F. （2） The optimal dose of"Bitongling" optimized prescriptionis made up of A₂B₁C₁D₁E₁F₁. （3） The optimized prescription of "Bitongling" hastherapeutical effect on joint damage of CIA, It can dose-dependent restrain ungula’sswelling of CIA Jimpy mice. and restrain activated lymphocyte to release NO and IFN-γtransfusion degrade IL-1β、TNF-αand anti-câ…¡antibody, these effective may be one of theimportant component for "Bitongling" of RA’s therapeusis. Its effective target is to inhibitactivated lymphocyte’s transference. Accordingly reduce inflammation regionalinfiltration, but restraining lymphocytic activation is inaction.