Studies On Quality Control Of Dangguisini Decoction

Dangguisini decoction was reported originally in shanghanlun, written by ZhangZhongjing. This paper is about the extraction process and quality assessment andpharmacodynamics of Dangguisini decoction, which is composed of Radix Angelicae Sinensis,Radix Paeoniae AIba, Ramulus Cinnamomi, Herba Asari, Radix Glycyrrhizae, MedullaTereapanacis and Fructus Jujubae.Using water and ethanol of different concentrations as the extraction solvent, the bestextraction solvent was 85% ethanol by studying extractive yield and the contents of ferulicacid, paeoniflorin, cinnamic acid and glycyrrhizic acid. The optimal alcohol extractionprocess of Dangguisini decoction was studied by orthogonal design with extractive yield andthe contents of ferulic acid, paeoniflorin, cinnamic acid and glycyrrhizic acid fromDangguisini decoction as quality assessment indices. Three factors were studied in thisexperiment, including the ethanol consumption, duration of extration and times of extration.The results showed that the optimal extraction condition was 85% ethanol consumpted 8times the amount of material, refluxing for twice and 1.5 hour per time.RP-HPLC methods were established to simultaneously determine ferulic acid in RadixAngelicae Sinensis, paeoniflorin in Radix Paeoniae Alba, cinnamic acid in RamulusCinnamomi and glycyrrhizic acid in Radix Glycyrrhizae. The separation of fore markercompounds was achieved on a Hypersil C₁₈ column （250 mm×4.6 mm, 5μm）with gradientelution using solvents of acetonitrile and aqueous acetic acid. The detection wavelength wasset at 275 nm and the flow rate was 1.0 mL·min^-1. Assay was linear over the range 0.86～20.64 μg·mL^-1 for ferulic acid （r=0.9992）, 20.0～200μg·mL^-1 for paeoniflorin （r=0.9993）, 2.10～27.3μg·mL^-1 for cinnamic acid （r=0.9995）, 2.14～29.96μg·mL^-1 for glycyzzhizic acid （r=0.9997）.Average recoveries of samples were 96.2% （RSD=1.5%）, 97.3% （RSD=1.0%）, 94.0%（RSD=1.2%） and 98.1% （RSD=2.0%） for four constituents, respectively. Four herbs ofDangguisini decoction were indentified by TLC with the dots clear and reproducible. Theassay methods were accurate and with good reproducibility and provide a quantitative basisfor the quality assessment for Dangguisini decoction.