Association Analysis Of ADIPOQ, ADIPOR1and ADIPOR2Genetic Variants And Adiponectin Expression With Gastric Cancer Risk

Purpose Here we investigated the association between genetic variants of adiponectin(ADIPOQ) and its two receptors (ADIPOR1and ADIPOR2) and the risk of gastriccancer in the Chinese Han population, and furthermore investigated correlationsbetween these selected SNPs and expression of adiponectin in gastric cancerous tissueswith clinical chracteristics of gastric cancer.Methods In this study, we conducted a genotyping analysis for adiponectin and itsreceptors genes in311cases of gastric cancer and425controls from the Chinese Hanpopulation by Sequenom. We investigated the expression of adiponectin with clinicalcharacteristics using tissue microarray (TMA) and immunohischemistry (IHC)technologies containing243cases from311gastric cancer patients and20normaltumor adjacent tissues.Results we found association of the gene variants with tumor location. SNPrs16861205with the minor allele A in ADIPOQ, SNP rs10773989with the minorallele C and SNP rs1044471with the minor allele T in ADIPOR2showed associationswith decreased risks in gastric cardia cancer [P=0.024, OR=0.605,95%CI=0.390-0.938; P=0.015, OR=0.699,95%CI=0.522-0.935; and P=0.022, OR=0.703,95%CI=0.519-0.951]. ADIPOQ gene rs16861205with minor allele A showedassociation with increased risk in gastric body cancer [P=0.010, OR=1.821,95%CI=1.148-2.890]. Further stratified analysis of the patients indicated that there weresignificant correlations between these SNPs of three genes and clinical characteristics, including tumor location, invasion depth, TNM stages, alcohol intaking, salted food,family history and gender. IHC data showed the expression of adiponectin in primarytumor tissues was significantly lower than in normal adjacent non-cancerous tissues,and the expression of adiponectin was significantly correlated with gender(P=0.014),invasion depth (P=0.024), family history (P=0.019) and alcohol intaking (P=0.037).Conclusion We concluded that variants of adiponectin and its receptor genes hadsignificant association with the risk of gastric cancer subtypes. Lower expressions ofadiponectin in the primary tumor tissues indicated the protective effects whichadiponectin plays under physiological conditions.