Effects Of Metformin On Proliferation Of Osteosarcoma MG63Cells And Expression Of Protein Kinase B And Forkhead Box Transcription Factor O1

Background Metformin has been recognized as one of the most effective drugs among type II diabetes patients. It was widely popularized because of its significant effect, little side-effect and low-cost. Some foreign scholars reported the low incidence of breast cancer, prostatic cancer, gastric cancer and lung cancer could be observed among diabetes patients with long-term metformin treatment. This phenomenon could also be found in some other malignant cells. This thesis put forward the idea whether metformin could also have impact on proliferation of osteosarcoma cells, Cell counting kit-8(CCK-8) was used to test the proliferation of osteosarcoma cells treated with metformin, and the Akt、p-Akt、FoxO1、p-FoxO1expression was analyzed by using Western blotting. So we can provide the basis of the possible mechanism of metformin inhibiting proliferation of osteosarcoma cells.Objective To investigate the effect of metformin on proliferation of osteosarcoma MG63cells and Protein kinase B (Akt) and forkhead box transcription factor01(FoxO1) phosphorylation in Akt signal pathway, and to explore the possible mechanism of metformin inhibiting proliferation of MG63cells.Methods Osteosarcoma MG63cells were cultured with RPMI1640supplemented with10%fatal bovine serun (FBS) untill the cells reached70%confluence, and cultured continuously with50mmol/L FBS-free RPMI1640. Cell counting kit-8(CCK-8) was used to test the proliferation of osteosarcoma cells treated with metformin at12,24,36,48, and72h. After extraction of total protein, the Akt、p-Akt、FoxO1、p-FoxO1expression was analyzed by using Western blotting in MG63cells at24h.Results The proliferation of the MG63cells could be significantly inhibited by metformin in a time-independent manner, and protein Akt, FoxO1phosphorylation could also be inhibited after48h. The p-Akt expression in metformin and control groups was0.62±0.04, and1.33±0.16respectively (p<0.05). the p-FoxO1expression in metformin and control groups was0.82±0.02and1.71±0.05respectively (p<0.05). Conclusion Metformin can inhibit proliferation of osteosarcoma cells in vitro, which is associated with the dephosphorylation of Akt/FoxO1signal pathway.