Protecive Effects Of Total Flavonoids Of Epimedium Against QA-induced Toxicity In SH-SY5Y Cells

Objective:To investigate the protective effects of total flavonoids of epimedium on toxicity induced by QA in SH-SY5Y cells.Methods:Toxicity of QA in SH-SY5Y cells:Cultured SH-SY5Y cells were divided into six groups:control group, different concentrations of QA groups.0.5、1.0、2.5、5.0、10.0mmol.L-1of QA were incubated with SH-SY5Y cells for24h, MTT sssay was used to detect the cell viability.Protection effects of total flavonoids of epimedium on toxicity induced by QA in SH-SY5Y cells:Cultured SH-SY5Y cells were divided into six groups:control group, DMSO control group, model group, low concentration, moderate concentration and high concentration of total flavonoids of epimedium groups. SH-SY5Y cells were routinely cultivated and pretreated with different concentrations of TFE (0.125、0.25、0.50mg·L-1) for24h, then incubating with QA for24h. MTT assay was used to detect cell viability; Cell apoptosis was observed by Hoechst33258staining; SOD、GSH-Px activities and MDA content were measured by colorimetric method; The mitochondrial membrane potential (△Ψm) and intracellular free calcium concentration ([Ca2+]i) were monitored by FCM.Results:Toxicity of QA in SH-SY5Y cells:With the increase of concentration of QA, cell viability decreased gradually by MTT assay. Cell activity decreased significantly at1mmol.L-1of QA(P<0.01).Protection effects of TFE on toxicity induced by QA in SH-SY5Y cells:0.25、0.50mg·L-1TFE increased cell viability of SH-SY5Y cells; Reduced apoptosis cells; Improved SOD、GSH-Px activities and decreased MDA content; Increased the mitochondrial membrane potential and reduced intracellular free calcium concentration ([Ca2+]i).Conclusion:QA has toxic effects on SH-SY5Y cells, TFE has significant protective effects against QA-induced toxicity, decreases apoptosis of SH-SY5Y cells, the action mechanisms may be associated with resisting oxidative stress, decreasing the [Ca2+]i and up-regulation△Ψm.