Effects Of Alareiin On Subcutaneous Xenograft Of Human Endometrial Carcinoma And Its Expression Of TRB3in Nude Mice

ObjectiveHuman endometrial carcinoma models were established by subcutaneoustransplantation in nude mice.After establishing the model of human endometrialcancer xenograft in nude mice, the model mice were injected subcutaneously withdifferent concentrations of alarelin.To study the effects of gonadotropin releasinghormone analogue (GnRH-a) Alarelin on endometrial cancer xenograft in nude miceand the expression of TRB3.MethodsEndometrial cancer cell line HEC-1B,in vitro after4-6weeks old female nudemice inoculated in the right arm near the armpit skin,subcutaneous xenograft modelwas established.Removing two nude mouse who with the biggest and the smallestsubcutaneous xenograft,then thirty-two nude mice with subcutaneous xenograft wererandomly divided into four groups:the control group,the low dose alarelin group,themiddle dose alarelin group,the high dose alarelin group.They are injected drug a weeklater.The control group was injected into the muscle with normal saline in0.2ml.Similarly,the low, middle and high dose groups were intramuscularly injectedrespectively with alarelin of15,30,60ug/kg in0.2ml. Each mouse was treated onceper day with6weeks.To measure the size and volume of tumor every sevendays.After6weeks,we should execute the nude mice,remove the xenograft tumor andcalculate the volume of tumor,further calculate the inhibition rate of the tumor.Tojudge the effects of the different doses of alarelin on tumor mechanism and theinfluence of TRB3gene expression. Results1.As the dose of alarelin reduced,subcutaneous xenograft tumor growth curveslope was successively large,and the speed of growth was also quick,a statisticallysignificant difference(P<0.05).2.After six weeks of the treatment,the tumor volume of each group was asfollows:the control group:1404.04±109.09mm3;the low dose alarelingroup:1219.40±158.97mm3;the middle dose alarelin group:1052.78±146.88mm3;thehigh dose alarelin group:818.37±192.91mm3(P<0.05).Different doses of alarelinprevented the growth of tumors in nude mice,alarelin inhibited the tumor growth by12.90±%、25.05±0.08%、41.92±0.12%in alarelin group of the low dose,the middledose and the high dose,and the difference was significant between thegroups(P<0.05)．3.The hematoxylin-easin staining results shown that disorders of cell structuresarranged in the control group,high beam,nuclear large and deep dyed andnuclear-pulpratio,nuclear fission is more.The alarelin group tumor cell size is slightlylarger,less sparse arrangement,deep-dyed in tumor cells,nuclear fast shrinking.Thealarelin group shown significant necrosis mor-phology changes under a lightmicroscope;4.The immunohistochemical results shown that alarelin can decrease theexpression of TRB3.As the doses of alarelin increased,the expression of TRB3decreased gradually(P<0.05). The differences between any two groups of all thegroups were significant(P<0.05).Conclusion1.Alarelin can significantly suppress tumor growth dose dependently.2. It may be related to inhibite TRB3protein expression, to inhibit proliferationof cells，to achieve the purpose of inhibiting tumor.