The Effect Of Neurologic Recovery On Rats With MCAO Through Motor Relearning Programme That Is Different In The Intervention Time

Object:It has been demonstrated that motor relearning programme is effective on improving the clinical symptom of patients with stroke. It can improve neurologic impairment of the patients. And the intervention time of motor relearning programme is crucial and can directly influence rehabilitation outcome. However it has been disputed when is the optimal time to begin the programme. The present study aimed to investigate that when was the best intervention time of motor relearning programme.Methods:The middle cerebral artery of adult healthy male Sprague-Dawely rats was occluded with thread embolism for90min to induce middle cerebral artery occlusion (MCAO), while control rats were not occluded. After MCAO induction, the rats were randomly divided into3groups and1of them was trained with motor relearning programme. And the group trained with the programme was divided into3small groups, trained at a different beginning time. Bederson was used to assess the recovery of neural function. Brain tissue around the ischemia area was collected on day7,14,21. mRNA levels of cpg-15and NF-κB were determined by RT-PCR.Results:1.The neurological functional scores of rats with MCAO were apparently different from control groups. And the scores were the lowest in group1with the earliest intervention time. The difference was statistically significant (P<0.05).2.The levels of cpg15in different groups were diverse after operation. Compared with the control group at the same time, the levels of cpg15were increased in group with motor relearning programme and ischemia group. And compared with other groups at the same time, the levels of cpg15in group1were the highest. The difference was statistically significant (P<0.05).3.After the operation, the levels of NF-κB in different groups were difference. Compared with the control group at the same time, the levels of NF-κB were increased in motor relearning group and ischemia group. Compared with ischemia group at the same time, the levels of NF-κB were reduced in motor relearning group. Compared with other groups at the same time, the levels of NF-κB in group B were the highest. The difference was statistically significant (P<0.05).Conclusion:Motor relearning programme can improve the defect of neurological function, increase the levels cpg15, and reduce the levels of NF-κB. And it is the optimum to be given motor relearning programme3days after MCAO.