Protection Mechanisms Of EPA On Renal Tubular Epithelial Cells Injury Stimulated By Albumin

BackgroundAmong various kinds of chronic kidney disease (CKD), renal fibrosis is the common pathological basis and the leading cause of end-stage renal disease. Pathological changes of the renal fibrosis include glomerulosclerosis and tubule-interstitial fibrosis. Damage to the renal tubular epithelial cells (HK-2) can cause the secretion of pro-inflammation cytokine, profibrogenic cytokines, and matrix protein. These inflammatory agents strengthen the injury to HK-2. This vicious circle is the major reason for renal fibrosis. Factors such as integrin linked kinase, integrin β1and peroxisome proliferator-activated receptor y (PPARy) have a very close relationship with renal fibrosis.N-3polyunsaturated fatty acid (n-3PUFA) is necessary in mammals. It mainly comes from the food, including eicosapentaenoic acid and docosahexaenoic acid. Fish oil is extracted from deep-sea fish, and is rich in n-3polyunsaturated fatty acids. Besides the anti-thrombotic effect, vasodilation, blood lipoid adjustment and anti-tumor effect, fish oil has a renal protective effect.In this study, we investigated the effects of EPA on the expression of PPARy, ILK and integrin (31in HK-2stimulated by albumin and to explore its possible protective mechanisms.MethodsThe HK-2of logarithmic growth phase was used. After serum-free cultured for24h, the cells were synchronized in the Go period, and then divided into four groups:the control group; human albumin group (model group, albumin20.0mg/L); Albumin plus EPA (10μmol/L,100μmol/L) group. All the cells were cultured in37℃,5%CO2for24h,48h and72h.The expression of PPARy, integrin β1and ILK was determined by Western Blotting; The mRNA expression of PPARy, integrin β1and ILK were eterminated by RT-PCR.Results1. Effects of EPA on the expression of PPARy in HK-2stimulated by albumin.Albumin could inhibit PPARy protein and mRNA expression; EPA could promote the albumin stimulation HK-2PPARy protein and mRNA expression.2. Effects of EPA on the expression of ILK in HK-2stimulated by albumin.Albumin could promote ILK protein and mRNA expression; EPA could inhibit the albumin stimulation HK-2ILK protein and mRNA expression.3. Effects of EPA on the expression of integrin β1in HK-2stimulated by albumin.Albumin could inhibit integrin β1protein and mRNA expression; EPA could promote the albumin stimulation HK-2integrin β1protein and mRNA expression.Conclusion1. EPA can promote albumin-induced HK-2PPARy protein and gene expression.2. EPA can reduce albumin-induced HK-2ILK protein and gene expression.3. EPA can promote albumin-induced HK-2integrin β1protein and gene expression.