An Association Study Of NF-κB1Gene And CXCL12Gene Polymorphism With Primary Antineutrophil Cytoplasm Antibodies Related To Small Vasculitis In Guangxi Han Nationality

Objective： To investigate the association of NF-κB1gene promoter-94ins/delATTG polymorphism with Primary ANCA associated small vasculitis(AAV) in guangxi han nationality.Methods: NF-κB1-94ins/delATTG were analyzed by Polymerase chainrestricted fagments length polymorphism (PCR-RFLP) in87cases of AAVpatients were collected and101cases of normal beings were contrasted, clinicaland pathologic data of the patients with different genotype were compared. Results:1、 The frequency distribution of NF-κB1-94ins/delATTGgenotypes of87AAV patients were WW:37.93%,DD:16.09%, WD:45.98%,respectively; and frequencies of alleles W and D were60.92%、39.08%.Whilein normal beings,genotype frequencies were WW:35.64%,DD:20.79%,WD:43.56%，respectively; frequencies of alleles W and D were57.42%、42.57%；No significant difference was found in either genotype distributions or allelefrequencies between the patients and the control (P>0.05);2、The levels ofsystolic pressure,serum creatinine and fasting blood-glucose were significantlyhigher than control group（P<0.05）;3、In AAV group, there was no significantdifference in the morbidity of Clinical symptoms such as fever, joint pain amongthe three genotypes（P>0.05）;4、Significant difference was found in serumCRP、RF levels of the three kinds of genotyped patients(P<0.05),the levels ofserum CRP、RF in WW genotype were significantly decreased;5、Significantdifference was found in IgM levels of the three kinds of genotypedpatients(P<0.05), the levels of IgM in DD genotype were significantlydecreased;6、In AAV patients, whether three genotypes in AI or CI differenceshad no statistical significance（P>0.05）.Conclusion:1、NF-κB1-94ins/delATTG gene has three kinds of WW,DD, WD gene polymorphism in patients with AAV in Guangxi;2、In AAVpatients, NF-κB1-94ins/delATTG gene polymorphism may be associated withserum CRP, RF, IgM levels. Objective： To investigate the association of CXCL12-3’G801Apolymorphism with Primary ANCA associated small vasculitis (AAV) inguangxi han nationality.Methods: CXCL12-3’G801A were analyzed by Polymerase chainrestricted fagments length polymorphism (PCR-RFLP) in87cases of AAVpatients were collected and101cases of normal beings were contrasted, clinicaland pathologic data of the patients with different genotype were compared.Results：1、The frequency distribution of CXCL12-3’G801A genotypes of87AAV patients were AA:3.45%、GA:36.78%、GG:59.77%, respectively; andfrequencies of alleles A and G were21.84%、78.16%;While in normalbeings,genotype frequencies were AA:7.92%、GA:43.56%、GG:48.51%，respectively; frequencies of alleles A and G were29.70%、70.30%； Nosignificant difference was found in either genotype distributions or allelefrequencies between the patients and the control (P>0.05);2、In AAV group,there was no significant difference in the morbidity of Clinical symptoms suchas fever, joint pain between the type two alleles（P>0.05）;3、Significantdifference was found in serum WBC、 triglyceride levels of the type twoalleles, carry A type alleles patients group obviously higher than carry G allele(P<0.05);4、In AAV patients, The differences of two type alleles in CI havestatistical significance（P<0.05）, A allele carriers CI index is significantly lowerthan the G allele carriers. Conclusion:1、CXCL12-3’G801A gene has three kinds of AA,GG,GAgene polymorphism in patients with AAV in Guangxi;2、In AAV patients,CXCL12-3’G801A gene polymorphism may be associated with the WBC,serum triglyceride levels, pathological CI index.