| Traditional Chinese medicine (TCM) contains a variety of ingredients, its effectiveness is the result of the interaction of its various components. The current "Chinese pharmacopoeia" general evaluate the quality of Chinese medicinal materials in some marks. However, sometimes those marks are not effective components, so it is not enough to guarantee its quality only by detecting these marks components. Since fingerprint have better specificity, stability and repeatability, it is more reliable to evaluate the quality of TCM. Due to the polarity differences of chemical composition in TCM, the fingerprint of the sample extracted by using a single solvent can not fully reflect the quality of the TCM and its preparations. Therefore, the present paper investigated the different polarity fingerprint with different polarity extraction solvent, and compared the pharmacodynamics effects. Combining pharmacodynamics effect, study the spectrum-effect relationship based on fingerprint and they can provide scientific support for the quality control of Angelica sinensis.Angelica sinensis and Enrichment Angelica Pills containing coumarin compounds, this kind of component has light toxicity. There is no light toxicity in normal usage, when it was used for a large number or long-term, which will cause accumulation in the body, and result in toxic reaction. We must strictly control the content of this kind of compounds in Angelica sinensis and Enrichment Angelica Pills.There are four parts in this dissertation:Chapter one:Study on chromatography fingerprint of different extract parts of Angelica sinensis To research the chromatography fingerprint of10batches of Angelica sinensis with high performance liquid chromatography (HPLC) and gas chromatography (GC). The similarity software of Chinese medicine fingerprint of Computer Aided Evaluation System was adopted for the data analysis and the method evaluation. The results showed that established fingerprint of different parts of10batches of Angelica sinensis firstly identify seven main index components. The method was accurate and reliable. The fingerprint established can be used as the reference for quality control of Angelica sinensis and its preparations.Chapter two:Study on chromatogram-pharmacodynamics relationship of Angelica sinensis on the effect of replenishing blood Blood deficiency model of mice was copied by subcutaneous injection acetyl phenylhydrazine (APH). Mice in each group were perfused with different extracted parts of Angelica sinensis (drug dosage was2.4g/kg) at tenth day, once a day for10days. Compare the influence of different extracted parts of Angelica sinensis to RBC, Hb, PLT and thymus, spleen and weight changes of blood deficiency mice. The peak areas of each common peak from HPLC fingerprint were associated with the date of replenishing blood pharmacodynamics efficacy by using gray relation statistic was used to research the chromatogram-pharmacodynamics relationship. The results showed that the part of SCS has the better effect in replenishing blood. The contribution degree of the SCS to replenishing blood of each component were determined by correlation size, and ferulic acid made the largest contribution, but contribution of other components should not be ignored. In this paper, we research the relationship of the HPLC fingerprint and spectrum activity relationship, determine the material basis of the SCS for replenishing blood, and provide effective way to represent the spectral correlation effect.Chapter three:Empirical study of effects of Angelica sinensis on mice with qi deficiency Qi deficiency model of mice was set up by controlling diet and fatiguing. Compare the influence of different extracted parts of Angelica sinensis to the indicators, like the phagocytotic ability of mononuclear macrophage and immune organs of mice with symptoms of qi deficiency. The peak areas of each common peak from HPLC fingerprint were correlated with the nourishing qi pharmacodynamics efficacy. The gray relation statistic was used to study the spectrum-effect relationship. The results showed that the part which was extracted with70%ethanol (ethanol extract) from Angelica sinensis, has certain improvement on the qi deficiency symptoms. According to gray correlation analysis method, the contribution of various components in ethanol extract with nourishing qi effect was determined by the spectrum-effect relationship, the order (characteristic peak number):18>5>7>12>17>4>19>2>20>6>8>3>14>1>16>11>9>13>15>10, peak7,10,11,13,17,18and20are ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A, ligustilide. butylidenephthalide and levistilide A, respectively. The results also show that there is a certain correlation between HPLC specific chromatogram and nourish qi.Chapter four:Determination of light toxic compound coumarin in Enrichment Angelica Pills and Angelica Sinensis by UPLC The ultra performance liquid chromatography (UPLC) method was developed for the simultaneous determination of psoralen, xanthotoxin and bergapten of Enrichment Angelica Pills and Angelica sinensis. The samples were extracted with methanol. The separation of target compounds were performed on Waters ACQUITY BEH-C18(100mm×2.10mm,1.7μm), with a gradient acetonitrile-water solvent system at30℃, the flow rate was0.45mL/min, the detection wavelength was set at245nm and the injection volume was3μL. Results showed good linear ranges for psoralen, xanthotoxin and bergapten within certain ranges, with correlation coefficients (r) of0.9992-0.9996, the mean recoveries were in the range of96.20%-102.42%, with the relative standard deviation of1.49%-2.97%. The method was simple, rapid, accurate and reliable. The method is suitable for the determination of psoralen, xanthotoxin and bergapten. |
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