Effects Of Atorvastatin On MRNA And Protein Expression Of Adropin In Cultured HUVECS And RASMCS

Part I Effects of atorvastatin on mRNA and protein Expression ofadropin in cultured HUVECSObjectives:To evaluate the effect of atorvastatin on mRNA and protein expression of adropin incultured human umbilical vein endothelial cells.Methods:Each group of cultured HUVEC was incubated with atorvastatin of0.002umol/l,0.02umol/l,0.2umol/l,2umol/l and20umol/l for6hours,12hours and24hoursrespectively.The vigor of HUVEC was detected byMTT chromatometry. The expressionof adropin mRNA in HUVEC was detected by RT-PCR and the expression of adropinprotein in HUVEC was detected by ELISA.Results:1.The vigor of HUVEC,which was incubated with atorvastain of different concentrationsfor6hours, the group of20umol/l was higher than the control group (P<0.05) and theother groups were higher than the control group but without statistical significance(P>0.05). Culture for12hours, the groups of0.02umol/l、0.2umol/l、2umol/l、20umol/lwere significantly higher than the control group (P<0.05) and the groups of0.2umol/l,2umol/l,20umol/l were significantly higher than the group of0.002umol/l(P<0.05). Culturefor24hours， the groups of different concentrations were significantly higher than thecontrol group (P<0.05) and the groups of0.02umol/l,0.2umol/l,2umol/l,20umol/l weresignificantly higher than the group of0.002umol/l (P<0.05).Each group of cultured HUVEC was incubated with atorvastatin of the same concentration for6hours,12hours and24hours separately.The vigor of HUVEC,whichwas incubated with atorvastain for12hours and24hours groups were significantly higherthan the6hours groups(P <0.05).2. The adropin mRNA expression of the different concentrations groups were significantlyhigher than the control group (P <0.001) and the adropin mRNA expression of0.02umol/l,0.2umol/l,2umol/l,20umol/l groups were significantly higher than the group of0.002umol/l.（P <0.001）.The adropin mRNA expression of HUVEC,which was incubated with atorvastain for12hours and24hours groups were significantly higher than the6hours groups (P <0.05).3. The adropin protein expression of the different concentrations groups were significantlyhigher than the control group (P <0.001) and the adropin protein expression of0.02umol/l,0.2umol/l,2umol/l,20umol/l groups were significantly higher than the group of0.002umol/l （P <0.001）.The adropin protein expression of HUVEC,which was incubated with atorvastain for12hours and24hours groups were significantly higher than the6hours groups (P<0.001).4.The adropin protein expression of the different concentrations were positively correlatedwith the OD value (6h， r=0.471, P=0.049) vs (12h，r=0.756, P=0.756) vs (24h，r=0.725, P=0.725).Conclusions:1.Atorvastatin dose-dependently and time-dependently promoted the proliferation ofHUVEC.2.Atorvastatin dose-dependently and time-dependently promoted the adropin mRNA andprotein expression of HUVEC.3.There was a positive correlativity between the adropin protein expression and theproliferation of HUVEC. Part II Effects of atorvastatin on mRNA and protein Expression ofadropin in cultured RASMCObjectives:To evaluate the effect of atorvastatin on mRNA and protein expression of adropin incultured rat aortic smooth muscle cells.Methods:The aortic smooth muscle cells（RASMC）were cultured in vitro by tissue explants.The morphology of RASMC was studied by phase contramicroscope. The RASMC wasidentified by immunofluorescence and immunohistochemistry methods.Passage4-5cellswere used in experiment.Each group of cultured RASMC was incubated with atorvastatinof0.02umol/l,0.2umol/l,2umol/l and20umol/l for6hours,12hours and24hoursrespectively.The vigor of RASMC was detected byMTT chromatometry.The expression ofadropin mRNA in RASMC was detected by RT-PCR and the expression of adropin proteinin RASMC was detected by ELISA.Results:1.The vigor of RASMC,which was incubated with atorvastain of different concentrationsfor24hours, the groups of different concentrations were significantly lower than thecontrol group (P<0.05). and the groups of0.02umol/l,0.2umol/l were significantly lowerthan the group of20umol/l (P <0.05).The vigor of RASMC,which was incubated with atorvastain of the same concentrationfor12hours and24hours groups were significantly lower than the6h group (P <0.05).2. The adropin mRNA expression of the different concentrations groups were significantlyhigher than the control group (P <0.05) but the adropin mRNA expression of0.2umol/l,2umol/l,20umol/l groups were significantly lower than the group of0.02umol/l.（P <0.05）.Each group of cultured RASMC was incubated with atorvastatin of the sameconcentration for6hours,12hours and24hours respectively.The adropin mRNAexpression of RASMC,which was incubated with atorvastain for12hours and24hoursgroups were significantly higher than the6hours groups (P <0.01).3.Each group of cultured RASMC was incubated with atorvastatin of0.02umol/l,0.2umol/l,2umol/l and20umol/l for6hours,12hours and24hours respectively.The adropin protein expression of different concentrations groups were significantly higher than thecontrol group(P<0.05). but with the increase concentration the expression of adropinprotein become lower and lower（P<0.05）.Each group of cultured RASMC was incubated with atorvastatin of the sameconcentration for6hours,12hours and24hours respectively. The adropin proteinexpression of RASMC,which was incubated with atorvastain for12hours and24hoursgroups were significantly higher than the6hours group (P <0.001).4. The adropin protein expression of the different concentrations were positively correlatedwith the OD value (6h， r=0.694, P=0.012) vs (12h，r=0.697, P=0.002) vs (24h，r=0.826, P=0.001).Conclusions:1.Atorvastatin dose-dependently and time-dependently inhibited the proliferation ofRASMC.2.Atorvastatin could promote the adropin mRNA and protein expression of RASMC,butwith the increase concentration the expression of adropin mRNAand protein become lowerand lower.3.There was a positive correlativity between the adropin protein expression and the vigorof RASMC.