| Objective:With median nerve conduction velocity and nerve bundles discharge as an indicator to explore rabbit Neiguan electroacupuncture the quantitative criteria for the in-depth study of acupuncture at Neiguan anti-myocardial ischemic (MI) foundation.Method:This study consists of three parts:The first observation point off the electrical stimulation of the median nerve in the nerve fibers recorded conduction velocity change, and then observe the hand needle three basic techniques that twisting, lifting and thrusting, twirling touching median nerve when inserting acupuncture at Neiguan nerve bundle discharge peak potential number changes, the last observation when the median nerve within electroacupuncture Neiguan nerve bundle discharge peak potential number changes, and with the hand needle for comparison.Experiment one:Select rabbits30, anatomical their upper limbs, isolated median nerve, median nerve trunk cut in addition to the surrounding tissue outside, and then lift the skin made of trough-shaped, quickly filled with warm paraffin oil to protect the nerve to prepare electrodes record.the separation of the median nerve under the nerve trunk mat a piece of thin plastic sheet so that the neural stem isolated from the surrounding tissue, nerve bundles to the heart side hung a special silver recording electrodes, recording electrodes into mesial distal end and two pairs of silver electrodes, two pairs of silver electrodes in contact with the nerve valid reference electrode on the skin incision, and then the animal is grounded. With BL-420S Biological Experimental System Test median nerve fiber conduction velocity.Experiment two:Select30rabbits were divided into three groups, twirling group acupuncture, acupuncture mention plug group, hands touching the needle is inserted twirling group. Also find the median nerve trunk and cut, lift the skin made trough-shaped, quickly filled with warm paraffin oil to protect the nerve to prepare electrode recording. Support the nerve trunk of the median nerve on a piece of thin plastic sheet so that the neural stem isolated from the surrounding tissue, the heart side hung nerve bundles silver recording electrode, monopolar guide, reference electrode connected to the surgical incision through the skin animals ground. With0.30×40mm needle piercing in bilateral Neiguan0.5-0.8cm, respectively, around twisting, lifting and thrusting, twirling and simultaneously lift and thrust stimulus, time of1min, the stimulation frequency of1Hz, hand needle the frequency control using acupuncture manipulation metronome guide (set to1/s), with basically the same rhythm induced nerve bundles discharged. Explore three basic acupuncture techniques that twisting, lifting and thrusting, twirling touching the basic situation of the median nerve when inserting acupuncture at Neiguan nerve bundle discharge peak potential number change.Experiment three:experimental rabbits were60, which cut off after20median nerve stimulation intensity was observed to give a fixed-induced changes in the median nerve discharge, the20rabbits were divided into two groups, the upper limbs of rabbits given a fixed intensity electrical stimulation, that are5V and10V. Another40rabbits given a certain strength of upper limbs first electrical stimulation, recording its nerve conduction velocity, in view of the experiment (a) recorded in the four kinds of conduction velocity, thus corresponding to40rabbits were divided into four groups of10. In determining its nerve conduction velocity, based on the median nerve cut, and then give the appropriate stimulus intensity and recorded median nerve discharge induced changes.Results:Experiment one showed that the electrical stimulation off points can be recorded on the median nerve to four kinds of nerve fiber conduction velocity, respectively62.5m/s,41.67m/s,31.25m/s and25m/s, and by statistical found:①When the stimulus intensity is small, I can guide a faster nerve conduction velocity, when a large stimulus intensity, the boot instead of nerve conduction velocity was slower.②same nerve fiber conduction velocity in different rabbits who need not identical stimulus intensity.③with the same stimulus intensity on different rabbits led out of the median nerve conduction velocity of the fibers are not identical.Experiment two observations indicate that the three basic techniques of acupuncture that twisting, lifting and thrusting, twirling touching when inserting acupuncture at Neiguan median nerve can be recorded to the nerve bundle discharge phenomenon. And has the following characteristics,①on twirling results, suggesting that not every hand twisting the needle stimulation can cause nerve discharge, meaning each hand twisting the needle stimulation at acupuncture points are not always effective;②About mention interpolation results, suggesting that although the hand needle insertion provided to stimulate nerve discharge efficiency higher than that caused by twisting exciting, but can not guarantee every nerve stimulation can cause a discharge phenomenon;③results twirling touching plug on hand needle twist inserted touching nerve discharge efficiency turn caused stimulation compared to the simple twisting or lifting and thrusting to be higher, but still can not guarantee that every nerve stimulation can cause a discharge phenomenon.Experiment three observation results show that if the first determine the nerve fiber conduction velocity, can guarantee to experimental animals meridian electroacupuncture warmed the types of afferent fibers is fixed, and can ensure that every time the electroacupuncture can cause nerve discharge phenomenon.Conclusion:One, The electroacupuncture Neiguan median nerve in rabbits can be recorded to four kinds of conduction velocity, that is62.5m/s,41.67m/s,31.25m/s,25m/s.Two, With the same stimulation intensity, the median nerve of rabbits at different guided fiber conduction velocity are not identical.Three, Hand needle twisting, lifting and thrusting, twirling touching when inserting acupuncture Neiguan rabbits can be recorded in the median nerve to nerve bundles discharge changes, but can not guarantee that each operation can log into the nerve bundles discharged.Four, Electroacupuncture in rabbits given Neiguan fixed intensity in the median nerve can be recorded to the nerve bundle discharge changes, but can not guarantee that each operation can log into nerve bundles discharged.Five, In the nerve fiber conduction velocity is determined on the basis of giving the rabbit hole off the corresponding intensity electrical stimulation, in which the median nerve can be recorded to the nerve bundle discharge changes, and can ensure that each operation can log into the nerve bundles discharged. |
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