The Promoter Methylation And MRNA Expression Of Wif-1Gene In Renal Cell Carcinoma

Objective:Wif-1（Wnt inhibitory factor-1,Wif-1) is regarded as one of themost essential inhibitors in a Wnt signal transduction channel.There arenumerous domestic and oversea experiments, which indicate that an abnormalpresence of expression and methylation of the promoter region of Wif-1inabundance of malignancies, presenting that aberrant methylation of thepromoter region may arouse some alterations in tumor mechanism.There israre research on the gene in renal cell carcinoma.The experiment is appliedwith the methylation-specific polymerase chain reaction (Methylation-SpecificPCR, MSP) and reverse transcription-polymerase chain reaction (ReverseTranscriptase-PCR,RT-PCR), to investigate the methylation status of Wif-1gene promoter and its mRNA expression levels in Renal cell carcinoma (RenalCell Carcinoma,RCC) and normal kidney tissue. In the meanwhile, throughanalysing their relationship with the clinical data, a certain theoretical basis isestablished in terms of pathogenesis, clinical diagnosis and treatment ofkidney cancer.Methods：156cases of corresponding normal kidney tissues and the methylationstatus of Wif-1gene promoter is detected by utilizing methylation-specificPCR(MSP) method. Besides, the research is to analyse the effect of the genemethylation in development of Renal cell carcinoma and its relation withclinicopathological data.2The reverse transcription-polymerase chain reaction (ReverseTranscriptase-PCR, RT-PCR) was used to detect the relative expressionquantity of Wif-1mRNA in56cases of Renal cell carcinoma tissues andcorresponding normal kidney tissues. The experiment is further analyzed the relationship between the relative expression quantity of Wif-1mRNA andclinical data in Renal cell carcinoma tissues.3SPSS17.0was used to analyze the results of experiment.Results：1The analysis of methylation of Wif-1gene in56cases of Renal cellcarcinoma tissues and corresponding normal kidney tissues, and therelationship between methylation and chinical dataIt is observed that the percentage of methylation of Wif-1gene in RRCtissues is46.5%(26/56), which was obviously higher than that the percentageof corresponding normal kidney tissues,3.6%(2/25, P<0.05). Therefore, thehuge difference suggested a certain statistical significance. Nevertheless, thereis no connection between the methylation status of wif-1and chinical data,such as gender, age, various clinical stages, tumor diameter, etc.2The relative expression quantity of Wif-1mRNA in56cases of Renalcell carcinoma tissues and corresponding normal kidney tissues, and aconnection between the relative expression quantity of Wif-1mRNA andchinical data in RCC.The relative expression quantity of Wif-1mRNA in RCC tissues(0.65±0.17), which is lower than in corresponding normal kidneytissues(0.77±0.06)(P<0.05). Based on analysis of statistics, the two group dataindicate a meaningful comparison. However, there is rare link between thelevel of relative expression quantity of Wif-1mRNA and chinical data, such asgender, age, tumor diameter, etc.3In RCC tissues,the relative expression quantity of Wif-1mRNA inmethylated tissues is0.68±0.17, which is a little higher than in unmethylatedtissues0.63±0.17(P>0.05). However, there is rare meaning in the contrastwith the two types of statistics.Conclusions:1The rate of methylation of Wif-1gene in RCC tissues is46.4%(25/56),which seems to be significantly higher than that in corresponding normalkidney tissue3.6%(2/56). According to analysis of statistics, if P<0.05, then the variety of data means a certain significance. The outcome indicates thathypermethylation of the Wif-1promtor in RCC is a common phenomenon,which may participate into occurrence of pathogenesis of RCC.2The standard of the relative expression quantity of Wif-1mRNA inRCC tissues (0.65±0.17) is lower than that in corresponding normal kidneytissues(0.77±0.06)(P<0.05).Those data imply that the reduction of relativeexpression of Wif-1mRNA may involve in the process of RCC.3In RCC tissues, the relative expression quantity of Wif-1mRNA inmethylated tissues (0.68±0.17) is a little higher than in unmethylatedtissues(0.63±0.17)(P>0.05),which suggests the high methylation rate of Wif-1gene may be a common event in kidney cancer. Nevertheless, the highmethylation rate of Wif-1gene cannot lead to reduce the relative expressionquantity of mRNA, which will demand further research to test the realspecify causes.