The Role Of MiR-27a In Erythroid Cells Differeneiation

Objective: To investigate the miR-27a expression and the role of itstarget gene, CDC25B, in erythroid differentiation. We analysis the γ-globinexpression level after miR-27a and CDC25B siRNA transfection, and exploretheir mechanism in erythroid differentiation.Methods: we induce K562cells differentiation with hemin(30nM) atdifferent time points (0,3,6,12,24,48h), and then detected the miR-27aandγ-globin expression with real-time PCR. Subsequently, we usedDual-Luciferase assay to verify that whether CDC25B was the target gen ofmiR-27a. we also transfeted the mimics and inhibitor of miR-27a into K562cells respectively and detect CDC25B andγ-globin expression.Results: miR-27a andγ-globin increased after hemin primed at differenttimes in K562cell lines, CDC25B express conversely with the miR-27a.Dual-Luciferase assay results showd that miR-27a could bind to CDC25B3’UTR, those indicated that CDC25B maybe the target gen of miR-27a.CDC25B protein was repressed after miR-27a overexpression in K562cellline and increased when miR-27a was inhibited. All above indicated thatCDC25Bis the target gen of miR-27a.γ-globin also up-regulated afterCDC25B siRNA transfection.Conclusion: Based on these studies, miR-27a andγ-globin up-regulatewhile K562cell induced with hemin. Western blot and Dual-Luciferase assayresults prove that CDC25B is the target gen of miR-27a.γ-globin up-regulatedafter miR-27a mimics transfection, but the miR-27a inhibitor group increaseless comparing with the mimics group. those indicate that miR-27a play animportant role in erythroid differentiation.γ-globin also increases when knock-down CDC25B, indicating that CDC25B take part in the erythroiddifferentiation. All above may get the conclusion that miR-27a regulateserythroid differentiation through its target gen CDC25B. Our paperinvestigate the role of miR-27a in erythroid differentiation and would give usa newway in leukemia pathogenesis and therapy.