The Effects Of Moxibustion BL23and ST36on ICAM-1and VEGF Of Serum And Rheumatoid Arthritic Rats

Objective:1. Comparing moxibustion, Thermal stimulus (cigarette) and Ai oil groupxtract role in "Shenshu(BL23)" and “Zusanli”(ST36) of rheumatoid arthritis(rheumatoid arthritis, RA) rat model effect differences. Explore the the moxibustionplay efficacy factors.2. Observation of moxibustion on rheumatoid arthritis model in the rat serum andsynovial of VEGF, ICAM-1of RA, content of moxibustion treatment the mechanism toprovide the experimental basis for the moxibustion effect.Methods:100male Wistar rats adaptive feed after a week, according to the HealthStatistics random number table randomly selected20for normal group, the rest of therats with freund’s complete adjuvant combined with wind, cold, wet environmentestablish RA rats model[1], then randomly divided into model group, the moxibustiongroup, Ai oil group, cigarettes group,(n=20/group), Normal group and model group ratsdo not make any treatment, Moxibustion was applied to“Shenshu”(BL23)and“Zusanli”(ST36), Moxibustion with a specially designed cigarette type moxamoxibustion2cm away from the hole,20min moxibustion group,20minutes each time,period of treatment is15days; Ai oil groups in the corresponding acupuncture pointswith the oil, every acupuncture point0.05ml, apply area of Φ=5mm centered onacupoint skin, once a day. Treatment for15days.Cigarettes group, three-ring card in red(Φ=5mm) away from the hole at2cm suspended moxibustion. After the treatment,observe the change of plantar swelling degrees. using ELISA to detect the contentof serum VEGF and ICAM-1; With lateral spreads the membrane organization, withimmunohistochemical method (SP) detection of VEGF and ICAM1expression insynovial tissue. The whole experiment process follow the experimental animal ethicsrules.Results:1. The ankle of RA model rat is obvious acute inflammatory swelling, visiblepart of the forelimb contralateral limbs or ears, tail swelling or rheumatoid nodules, plantar circumference significantly increased compared with normal group (P﹤0.01);After15days after treatment, moxibustion group rat and cigarette group plantarcircumference were less than model group (P﹤0.01), but compared with the normalgroup there are still differences (P﹤0.05); Rat plantar circumference of artemisia oilgroup compared with model group there was no significant difference (P>0.05);Moxibustion group rat plantar circumference less than cigarettes (P﹤0.05).2. Rheumatoid arthritis in rats serum VEGF and ICAM-1levels increased significantlycompared with normal group (P﹤0.01); Moxibustio and cigarettes group VEGF、ICAM-1content significantly lower than model group (P﹤0.01), but compared withthe normal group, there is still a significant difference (P﹤0.01); Ai oil group of VEGFin serum of rats, ICAM-1content compared to model group there was no significantdifference (P>0.05); Moxibustion group rats serum ICAM–1and VEGF contentlower than cigarettes group (P﹤0.05).3. The VEGF in synovial tissue location in the cell membrane and cytoplasm, to appearbrown granules for positive response. Also is a small amount of VEGF expression innormal group of synovial tissues; Model group synovial tissue VEGF expressionincreased than normal group (P﹤0.01); Moxibustion group VEGF expression insynovial tissues of rats decreased significantly than model group (P﹤0.01), comparedwith normal group, still had significant difference (P﹤0.05); compared with modelgroup, Cigarette group in VEGF expression also decreased obviously (P﹤0.01), butcompared with the normal group, there is still a significant difference (P﹤0.01); Ai oilgroup in synovial tissue VEGF expression in the rat model group have no significantdifference (P>0.05); compared with cigarette group, the expression of synovial tissueVEGF in Moxibustion group were decrease (P﹤0.05).4. The ICAM-1in synovial tissue location in the cell membrane and cytoplasm, toappear brown granules for positive response. Also is a small amount of VEGFexpression in normal group of synovial tissues; Model group ICAM-1expression insynovial tissues increased compared with normal group (P﹤0.01); Moxibustion groupof ICAM-1expression in synovial tissues of rats decreased significantly compared withmodel group (P﹤0.01), compared with normal group, there was no significant difference (P>0.05); compared with the model group Cigarette group of ICAM-1expression in synovial tissues of rats also significantly lower (P﹤0.01), but comparedwith the normal group, there is still a significant difference (P﹤0.01); compared withmodel group,Ai oil group of ICAM-1expression in synovial tissues of rats have nosignificant difference (P>0.05); compared with cigarette group, the expression ofsynovial tissue ICAM-1in Moxibustion group were decrease (P﹤0.05).Conclusion:1. Moxibustion "Shenshu"“Zusanli”can significantly relieve rheumatoidarthritis rats ankle swelling.2. Moxibustion can reduce rheumatoid arthritis in rats serum levels of ICAM-1, reduceICAM1expression in the synovial tissue, reduce early variety of the release ofinflammatory cytokines, infiltration, and cross endothelial mobile, thus reducingsynovial inflammation, improve local symptoms.3. Moxibustion can reduce rheumatoid arthritis in rats serum VEGF levels, reduce theexpression of VEGF in synovial tissue, stop the angiogenesis, pannus formation, thusimproving synovial inflammation.4. Improve local swelling, decrease in serum levels of VEGF, ICM-1and itsexpression in synovial tissue, moxibustion, cigarettes is better than Ai oil group, promptwarm stimulation produced by moxibustion is one of the factors to improve the localsymptoms of rheumatoid arthritis rats, and moxibustion can inhibit VEGF levels, ICAM-1, improvement of local tissue adhesion, joint symptoms such as swelling, adverseevents, this part provides experimental basis for the warming through effect ofmoxibustion.