Study Of Auricularia Auricular Polysaccharides’ Effect On Cerebral Edema Of Intracerebral Hemorrhage Rat Model

ObjectiveWe aim to investigate the protective effect of auricularia auricularpolysaccharide on cerebral edema of intracerebral hemorrhage rat model byobserving the changes of neurological severity coloboma score, water contentof brain tissue, brain tissue’s histopathology and blood-brain barrier permeability72hours after intracerebral hemorrhage.Methods80male SD rats were randomly grouped as sham group, model group, lowor high dose group. The low or high dose groups’ rats were intragastricadministrated with100mg/kg or500mg/kg of auricularia auricularpolysaccharide once a day for14days. Sham group and model group weregiven equal volume of normal saline once a day for14days. Then the rats wereinjected with2μl normal saline solution containing0.4U collagenase Ⅶ into rightcaudate nucleus to establishing intracerebral hemorrhage model. Sham group’srat performed the same steps as above but without collagenase Ⅶ in normalsaline solution. Seventy-two hours after operation the rats were separated into2sub group A and B. Rats in sub group A were executed and the brains weretaken from the rats. For measuring variation of water content in brain tissue, theright half brain was coronarily separated into anterior part and posterior part.The anterior part was used to test the water content in brain tissue. The posterior part was made into sections for HE stain to investigatehistopathological changes by light microscopy. Rats in sub group B wereinjected with EB solution by tail vein and were killed after2hours. Thenapproximately100mg brain hematoma tissue were separated to determine thepermeability of the blood-brain barrier through the detection of brain tissuetracer EB content. All data were expressed as Means±SD deviation, andprocessed with SPSS16.0.ResultsFour out of80rats were died after operation. Seventy-two hours afteroperation, sham group’s neurological function coloboma score, water content ofbrain tissue and the content of EB were remarkable lower than model group’s（p<0.05）. Tow dose groups’ above data were lower than the model group’s（p<0.05）. High dose group’s were significantly lower that low group’s（p<0.05）.Observation of HE stained showed that parts of nerve cells appeared mildedema in sham group; some had mild edema around blood vessels. Braintissue around hematoma in model group showed addition of water quantity andedema obviously and around the hematoma in rats were osteoporosis andlightly stained which caused a large number of inflammatory cell infiltration andmade neurons necrosis and degeneration. In low does group there was mildedema around hematoma. There were a modest number of inflammatory cellsinfiltration. The high doses group’s were of a small scale. There was noapparent mild edema with a little number of inflammatory cell infiltrations. Theseresult showed the addition of model group in water content of brain tissue,raising in neurological severity coloboma score; the aggravation ofhistopathology and the rise of EB content, so the model group were successfullyestablished. The dose groups had given relief to the damage with the increaseof neurological function coloboma score, the rise of brain tissue water content,the damage function of blood-brain barrier, which caused by intracerebral hemorrhage. Higher dose of auricularia auricular polysaccharide brought bettereffects.ConclusionEstabalishment of ICH rat model by collagenase injection was easy toperform and the results was reliable. The auricularia auricular polysaccharidecould reduce the brain damage caused by intracerebral hemorrhage, reduce thebrain edema and release neurological function coloboma. Neuro-protectiveeffect of higher dosage of auricularia auricular polysaccharide administation wasbetter than low dosage.