Studies On Serum Metabolomics Of Osteoporosis Rats And The Anti-osteoporosis Effect Of Eucommia Ulmoides Oliver Small Compound

Osteoporosis is a metabolic bone disease characterized by low bone mass, impaired micro-architecture and susceptibility to fracture. It’s often occurred in old people and postmenopausal women. Bone mineral density (BMD) as a "gold standard" has been used in predicting the risk of fracture for a long time. However, alterations in bone mineral density are slow in the dynamic disease progress of osteoporosis. As the slow change and slow sensitivity of BMD, there isn’t a biomarker which could reflect the progress of OP objectively and accurately. Metabolomics is the study of all the changes in metabolites generated by environmental or genetic changes. Osteoporosis model and GC-MS were used to construct the metabolic profiles of serum metabolites. Then chemometrics methods, such as pattern recognition, were adopted to make a comprehensive metabonomics study to search the objective and accurate biomarkers which could evaluate the change of OP after treatement.Research on the content of this paper is mainly referred to the following two parts:1、Silanization derivative method combined with GC/MS was applied to analyze the OP serum metabolites in rats.Most serum metabolites were derivated and59of them were qualitatively analyzed. The whole metabolic characteristics of OP rats were obtained. Simultaneously, the applied GC-MS method was validated with acceptable precision, lineary and repeatability.2、The Principal Component Analysis (PCA) and Partial least squares method-discrimination analysis (PLS-DA) methods combined with the obtained metabolic profiling were employed to build up the discriminant model between healthy controls and OP rats.5metabolites were selected as the potential biomarkers in mice serum via Subwindow Permutation Analysis(SPA).The method developed has provided valuable information for exploring the pathogenesis, and evaluation of OP after treatment. Simultaneously the5makers were also applied to evaluate the progress of OP after E2and Eucommia ulmoides Oliver small compound treatment. The metabolomics was used to assess the anti-osteoporosis effect of Eucommia ulmoides Oliver small compound.