| purpose:To observe perfusion process and enhance characteristics of VX2hepatic tumor in rabbits by using contrast enhanced ultrasound (CEUS), investigate pertinence between all quantitative indexes of time-intensity curve (TIC) and tumor vascular endothelial growth factor (VEGF) and explore application value of each index in VX2hepatic tumor in rabbits.Methods:Twenty-five healthy New Zealand white rabbits were allocated randomly in trial group with twenty-two rabbits and control group with three rabbits. The VX2hepatic carcinoma mass were implanted into the liver of rabbits in trial group via laparotomic route. The rabbits were performed by CEUS after2weeks. Data of TIC within tumor and surrounding hepatic tissue were read, including AT, TTP, PI, AUC, etc. Relative differences, namely relative AS, relative PI, relative TTP, relative AT and relative AUC were calculated. We used streptavidin-perosidase to detect VEGF expression and analysed the pertinence between VEGF and the above data.Results:1.19out of22experiment rabbit cases were successfully patternmade and the success ratio was86.4%.2. There were significant differences (p<0.001) between VX2hepatic tumors and surrounding hepatic tissue of the same depth for AT, AS, TTP, PI and AUC.3. For all relative difference, of TIC quantitative indexes, relative TTP was positively correlated with the expression of VEGF (p<0.001), while other indexes did not show significant difference.Conclusion:1. VX2tumor tissue implantation to make models is convenient, feasible, time saving and provides a more suitable model for surgical or interventional studies.2. All quantitative indexes of CEUS showed significant difference between VX2tumor and the surrounding normal liver(p<0.001), which provides strong evidence in evaluating hepatic tumor blood perfusion.3. The relative TTP was positively correlated with expression of VEGF, which is meaningful to some extent in evaluating angiogenesis of hepatic VX2tumor vessels in rabbits. |
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