Expression Of CCND1,ORAOV1, ERCC1and Their Significance In Squamous Cell Carcinoma Of Tongue

ObjectiveTo study the expression of CCND1, ORAOV1, ERCC1genes in tongue squamous cell carcinoma, investigate the relationship between CCND1,ORAOV1, ERCC1and the occurrence of tongue cancer as well as their clinical significance.MethodImmunohistochemistry staining were applied to determine the expression of CCND1,ORAOV1and ERCC1in tissue microarrays of38tongue squamous cell carcinoma patients and4cases of normal tongue tissue. Semi-quantitative and qualitative method were used to study the occurrence and development of CCND1,ORAOV1, ERCC1in tonguecancer.Result(1) The proportion of CCND1expression in tongue squamous cell carcinoma and in tongue normal tissue were73.68%and25.00%, respectively. The comparison between2groups had significant statistical difference (p<0.05). The proportion of CCND1over-expressed in squamous cell carcinoma was28.94%while there was no over-expression in normal tissue. The comparison between2groups had no statistically significance (p>0.05).(2) The proportion of ORAOV1expression in tongue squamous cell carcinoma and in tongue normal tissue were84.21%and25.00%, respectively. The comparison between2groups had significant statistical difference (p<0.05). The proportion of ORAOV1over-expressed in squamous cell carcinoma was42.10%while there was no over-expression in normal tissue. The comparison between2groups had no statistically significance (p>0.05)。But comparing the over-expression of ORAOV1in difference histopathological differentiation we found statistically significance (p<0.05). (3) The proportion of ERCC1expression in tongue squamous cell carcinoma and in tongue normal tissue were60.52%and100.00%, respectively. There was no statistical difference when comparing two groups (p>0.05). The proportion of ERCC1over-expressed in squamous cell carcinoma was23.68%while in normal tissue was75.00%; the comparison between2groups had significant statistical difference (p<0.05). When comparing the over-expression of ERCC1in difference histopathological differentiation we found statistically significance (p<0.05).Conclusion(1) In tongue squamous cell carcinoma group CCND1showed up regulated expression suggesting that CCND1may be closely associated with the occurrence of tongue cancer.(2) In tongue squamous cell carcinoma group ORAOV1showed up regulated expression and the over-expression of ORAOV1showed up regulated associated with histopathological differentiation suggesting that ORAOV1may be closely associated with the occurrence of tongue cancer.(3) In tongue squamous cell carcinoma group ERCC1showed down regulated expression and the over-expression of ERCC1showed down regulated associated with histopathological differentiation suggesting that ERCC1may be closely associated with the occurrence of tongue cancer.