Expression Of MiRNA-125b In Breast Carcinoma And Its Clinical Significance

Objective:To examine the expression level of miRNA-125b in breast cancer tissues and corresponding normal tissues adjacent to carcinoma and breast benign lesions by quantitatively detecting and analyze its correlation with clinical pathological features and significance.Methods:Adopt the method of real-time qPCR to dectect the expression of miRNA-125b in breast cancer tissues and its corresponding normal tissues adjacent to carcinoma from34patients and breast benign lesions from10patients. Analyze the relationship by SPSS18.0software between their expression and clinical pathological features, such as patient’s age, tumor size, status of estrogen and progesterone receptor, cerbB-2, histological grade, lymph node metastasis, proliferation index Ki-67, etc.Results:Expression of miRNA-125b in breast invasive ductal carcinoma is lower than non-tumor tissues (normal tissues adjacent to carcinoma and breast benign lesions)(P=0.003;0.031).That expression of miRNA-125b doesn’t have obvious difference between normal tissues adjacent to carcinoma and breast benign lesions (P>0.05).Abnormal expression of miRNA-125b was significantly associated with cerbB-2(P =0.019), lymph node metastasis (P=0.014) and tumor size (P=0.012) and cell proliferation index Ki-67(P=0.023); There is not statistical significance in histological grade, status of estrogen and progestational hormone receptor and tumor clinical stage.Conclusions:(1) Compared with the normal tissues adjacent to carcinoma and benign lesion group, low expression of miRNA-125b in breast invasive ductal carcinoma indicates that miRNA-125b may play the role of tumor suppressor.(2) Down-regulation of miRNA-125b correlates with overexpression of cerbB-2, lymph node metastasis, tumor size and proliferation index Ki-67.So miRNA-125b may be a new tumor marker for prognosis judgment of breast cancer patients, providing a new target for the treatment of breast cancer.