The Application Value Of T-SPOT TB In The Diagnosis Of Tuberculous Pleurisy

Tuberculous pleurisy is the most common extrapulmonary tuberculosis, andis also the most common cause of pleural effusion.Because of the lack ofspecific clinical manifestations,Traumatic pleural biopsy limits its clinicalapplication,Molecular biology, providing rapid diagnostic techniques,still hasissues in false positives and technology which brings some difficulty to the earlydiagnosis of tuberculous pleurisy,T-SPOT TB,rising in recent years, is used asa method to detect cell immune function,This method is of high sensitivityand specificity，and Gradually become hot spot of TB diagnosisresearch.This study is based on the detection of peripheral blood T-SPOT TBfor93cases of hospitalized patients with pleural effusion to explore the T-SPOTTB in tuberculous pleurisy in clinical applications.Objective:Through the peripheral blood in patients with pleural effusion T-SPOT detection of TB,With pleural effusion of adenosine deaminase (ADA),serum tuberculosis antibody detection, water chest n/med tuberculosis bacteriumculture comparison,To explore the value of T-SPOT TB mycobacteriumtuberculosis (T cell SPOT test technology) in the diagnosis and differentialdiagnosis of tuberculous pleurisy in the clinical application.methods:The subjects were93cases,48patients with tuberculous pleurisy,45cases of malignant pleural effusion.With T-SPOT TB testing thesubjects of peripheral blood mononuclear cell (PBMC) of mycobacteriumtuberculosis in early production target antigen6(ESAT-6) and/or culturefiltrate protein (CFP) sensitization10T lymphocytes (namely spots formingcells, SFC), the number of,And other related indicators: pleural effusion ofadenosine deaminase (ADA), serum tuberculosis antibody detection, water chestn/med tuberculosis bacterium culture were compared.Results:1,T-SPOT TB testing positive rate was91.67%in group oftuberculous pleurisy,Is significantly higher than the positive rate of malignantpleural effusion were:8.89%, the difference is statistically significant (P <0.05)2, combined use of early production target antigen6(ESAT-6) and culturefiltrate protein (CFP) the sensitivity of10(91.67%) is higher than use ESAT-6sensitivity (85.42%) or use the sensitivity of the CFP-10(75.00%),But thedifference between combination and use either the sensitivity of the antigen hasno statistical significance.3, T-SPOT TB, pleural effusion adenosine deaminase (ADA) detection,serum tuberculosis antibody detection, hydrothorax cultivate the sensitivity ofn/med tuberculosis bacterium are91.67%,70.83%,62.50%,70.83%,Specificityare91.11%,55.56%,62.50%,91.11%, the diagnostic accuracy rate are94.24%,63.44%,62.37%,63.44%,T-SPOT detection sensitivity is higher than Othertest methods, the difference is statistically significant (P <0.05).Conclusion:T-SPOT TB can be used for the diagnosis and differential diagnosis of tuberculous pleurisy.In the diagnosis of tuberculous pleurisy in T-SPOT TB has higher sensitivity, specificity and diagnostic accuracy,T-SPOTTB on the early diagnosis of tuberculous pleurisy has high application value,andis worth in the clinical application and spread.