Preliminary Research On The Expression Of SATB1、Wnt1and β-catenin In Breast Cancer Stem Cells

Background:SATB1(special AT rich sequence binding protein) regulates the expression ofmany genes.The expression changes of the genes which are induced by SATB1consist with epithelial-mesenchymal transition (epithelial to mesenchymal transition,EMT) characteristics,which can make the mammary gland epithelial cells obtaine thefeature of the stem cells.Wnt/β-catenin signaling pathway also plays an importantrole in the development of breast cancer.This signaling pathway regulatesself-renewal and differentiation of cancer stem cells. Being a vital member of Wnt/β-catenin signaling pathway,β-catenin is important to the transduction of this signalingpathway.This study intends to compare the expression differences of SATB1、Wnt1andβ-catenin in breast cancer cells and breast cancer stem cells,preliminarly discussthe significance of the expressions in breast cancer stem cells.Methods:1、Cultivating MCF-7cells of breast cancer and enriching breast cancer stemcells by suspension culture; UsingFlow cytometryto identify breast cancer stem cells;2、Detecting the expressions of SATB1, Wnt1and beta-catenin in breast cancercells and breast cancer stem cells by RT-PCR;Calculating the relative expressions ofmRNAs.The results were statistically analyzed by SPSS19.0statistical software.Results:1、 Constructed target genes recombinant plasmid and standard curve;2、Compared to breast cancer cells, the expression of SATB1in breast cancerstem cells was significantly increased, the difference was statistically significant (P<0.05);3、The expression of Wnt1in breast cancer stem cells was significantly higherthan which was in breast cancer cells(P <0.05);4、The expression of β-catenin in breast cancer stem cells and in breast cancercells showed no significant difference (P>0.05);Conclusions: The expressions of Wnt1and SATB1in breast cancer stem cells were higherthan them in breast cancer cells,prompting that SATB1and Wnt/beta-cateninsignaling pathway may play a role in the regulation of self-renewal of breast cancerstem cells.The results and the methods established in this study made a foundation forfurther study.