Effect Of Toufeng Decoction On The Expression Of C-fos、c-jun Gene In Peiraqueductal Gray Of Migraine Rat Model

BACKGROUND:Migraine is a primary nervouse system dysfunction disorder, whichperforms recurrent one or both sides throbbing headache.the seriouse one can lose theability of daily life. With the increasing incidence year by year,Migraine brings gravephysical、mental and economic burden to patients,.therefore,,Global medical communityhas being focusing on the migraine’s prevention and treatment,which becomes an urgentobligation.It’s mechanism is quite complication,having not been illustrated completely. Inthe preliminary,the head wind soup making by two chen shang flavored has made satisfiedresults based on the principle of heat clearing and damp drying. The periaqueductalgray(PAG) is a area whose function and structure is relatively complex,.It wideningparticipate in the regulation of the body function such as painting regulation、fear anxietyand autonomic nerve response,which is considerated as the pain adjusting center.PAGplays an essential role in pain modulation process.A mass of research statistics indicatesthat PAG is bound with migraine,but it’s effect is not definite..The c-fos and c-junproto-oncogene belongs to immediate early gene.They play an important role in the singnalconduction and control process of the central nervous system activation.The two geneshave being widely used as an symbol of the stimulation of neurons.and the study of neuronfunctional pathways in the various kind of migraines. Multiple of researches find IEGcontributes to the occurrence of migraines greatly.Objective: Set up the migraine models induced by nitroglycerin. With theimmunohistochemical method, Observe the expression of c-fos and c-jun in the rat PAG ofmigraine models and the effect after drinking the head wind soup.Discuss the role of PAG in the occurrence of migraine. and the relationship of c-fos and c-jun. Clarify themechanism of treating migraine by head wind soup from the molecular biology.. Testifythe availability of prevention and therapy in the migraine.futhermore and supply newthinkings for chinese traditional medicine.METHOD: Divide80SD rats into4groups,including normal control group、modelgroup、 head soup soup treatment group,、head wind wind prevention group. Every groupcontains20rats, ten is used to observe the behavior, another10are killed and remove thebrain,then make immunohistochemical staining.The nomal control do not do anyprocession.Set up experimental migraine model by injecting NTG at the dose of10mg/kgunder subcutaneous in the model group、head soup soup and treatment group. Observe thebehaviors of rats. Pour distilled water into the somach in the model group after makingmodels. Pour head wind soup at the dose of0.24ml/g into the somach in the treatmentgroup after bulding30min. Perfusate head wind soup at the dose of0.24ml/g a week intothe somach in the preventing group before making models After buiding models4,putbrain out of the head and then slice、prepare speciments.Observe the positive cell numbers、area and gray that c-fos and c-jun express in the PAG of each group by usingimmunohistochemistry ABC.RESULTS:We succeed in repeating mingraine rat models after injecting NTG.,eachexperimental rat display typical behavior such as red ears,scraching head and climbinghutch. the positive cells of the expression of c-fos and c-jun in brain tissues increase inthe model group. There is significant statistical difference compared with normal controlgroup(P<0.05),the expression of c-fos and c-jun has significant correlation(r=0.989，P<0.05). the positive cells of the expression of c-fos and c-jun in brain tissues increase inthe treatment and preventing group significant reduced, There is significant statisticaldifference compared with model group(P<0.05),and no statistical difference comparedwith normal control group(P>0.05).Conclusion: we succeed in inducing the migraine rat model by injecting NTG, whichkind of migraine model is regarded as an steady、efficient and economic experimentalmethod.PAG plays an important role as pain adjusting center when migraine attacks.Theexpression of c-fos and c-jun are significantly higher when migraine breaks out.,which isan symbol of neuron activation. PAG is vital in algogenesis and signal transmission.Thehead wind soup can largely low the expression of IEG, relieve the behaviors of migraine rats. The head wind soup can effectively prevention and cure migraine.Heat-clearing anddamp-dying is an considerable way to treat migraine.