| More and more incidence of malignant tumors, radiotherapy andchemotherapy is the common treatments, but two ways to kill tumor cellswhile also damage normal tissue organ, which may result the adverseevents, bone marrow suppression is a frequent adverse reaction. Most ofthe anticancer drugs with bone marrow suppression, first expressed as thetotal number of leukocytes (total white blood cells, WBC) reduction andneutropenia, followed by a decrease in platelet (the Platelets PLT) andbleeding tendencyserious may be white blood cells, red blood cells (redblood cell, RBC) and PLT reduce.For neutropenia reduce medication,which the most commongranulocyte colony-stimulating factor (Cranulocyte-colony stimulatingfactor, CSF) CSF may be effective for most patients with leukopenia, butstillis invalid, still has some adverse effects, and expensive.Therefore, theneed to find more effective and/or side effects are smaller and/or cheapertreatment. In recent years, low-intensity pulsed ultrasound (Low-Intensity PulsedUltrasound, LIPUS) as safe method physiotherapy, and gradually applied toclinical treatment, such as promoting the healing of bone and soft tissuetrauma. by its means of transmission of the pulse, the dose can be as low asbelow30mW/cm2, simple, safe, effective and inexpensive. The found thatthe LIPUS can promote the proliferation of a variety of cells, includingfibroblasts, mesenchymal stem cells.Our previous study the LIPUS parameter of filtered out the suitabilityfollow-up experiments, and initially confirmed the after LIPUS radiationafter, normal bone marrow nucleated cells proliferation faster, the numberof cells increased significantly.And bone marrow nucleated cellsproliferation closely related with the increase in WBC.This subject basedon experimental to continue in-depth study,refer to rats and miceLeukopenia Animal Modelused cyclophosphamide establish New Zealandrabbit leukopenia model.LIPUS treatment model animals to observewhether elevated WBC,to explore the possibility of LIPUS for thetreatment of leucopeniaObjective1Determination of normal rabbit blood of New Zealand,to establish themodel of the New Zealand rabbit leukopenia by cyclophosphamide; 2Contrast observation New Zealand rabbit’s ships and its bloodchanges after using the CSF and LIPUS irradiated for short-term (7days);3Observed a longer period of time (40days) using LIPUS, The NewZealand rabbit general and its blood changes.Method1150New Zealand rabbit ear vein injection of cyclophosphamide to doseto50mg/kg injection of4consecutive days. During the injection period andafter injection, Extract0.5-1ml blood of the rabbit ear artery,to bloodroutine examination.2Split the60rabbits into3groups equally, this indicates the LIPUS, GSFand Control group: The LIPUS group: taking the condition of radiation of6W/cm2,20min with days. Checking the condition of drinking,feeding, mood,pissing activities and live or not. Getting the routine blood test per2days.getting the pathological section from skin and muscle;The GSF group:injecting the recombinant human granulocyte colony-stimulating factor asdosage of5μg/kg without interrupt in7days;The control group: without anytreatment;3Split the60leukocyte decrease rabbits into2groups equally: LIPUSand s control group. The LIPUS group: continue to radio as0.6W/cm2，20min without interrupt40days; the control group: without any activities. Result1New Zealand rabbit after injection of cyclophosphamide diarrheaadverse effects and even death.During the observation period, a total of95diarrhea, diarrhea was63.33%;30died,the mortality was20%.24hoursafter injection of cyclophosphamide, WBC reduced to four days to a lowvalue (2.37±1.03×109/L), the administered former WBC20%;22days,is still at a low level (4.90±0.54×109/L), the administration of formerWBC42%,4and22days the WBC before administration,WBC decreasewas statistically significant (p <0.05).2LIPUS group and CSF group rabbit model of diarrhea after treatmentbegan to improve the LIPUS group of diarrhea and death occurs mainly inthe start of irradiation, the7th day is no longer diarrhea and death,a total of11models rabbit diarrhea, diarrhea was55%;2died, the mortality rate was10%. CSF group, diarrhea and death occurs mainly in stop using the CSF, atotal of12models rabbit diarrhea, diarrhea was60%; death five mortalityrate was25%. Between the two groups of diarrhea and mortality rates werenot statistically significant (p>0.05). Diarrhea and death of the controlgroup there has been of diarrhea17only, diarrhea was85%;14deaths only,the mortality rate was70%. LIPUS group rate of diarrhea and mortalitycompared with the control group, were lower than the control group,astatistically significant (p <0.05); CSF group compared with the control group, diarrhea rate was higher, but not statistically significant;mortalityrate higher than that of the control group, and was statistically significant(P <0.05).LIPUS group and CSF group rabbit model of diarrhea after treatmentbegan to improve the LIPUS group of diarrhea and death occurs mainly inthe start of irradiation, the7th day is no longer diarrhea and death occurred,a total of11rabbit model diarrhea, diarrhea was55%;2died, the mortalityrate was10%. CSF group, diarrhea and death occurs mainly in stop usingthe CSF, a total of12models rabbit diarrhea, diarrhea was60%; death fivemortality rate was25%. Between the two groups of diarrhea and mortalityrates were not statistically significant (p>0.05). Diarrhea and death of thecontrol group there has been of diarrhea18only,diarrhea was90%;16deaths only, the mortality rate was80%. LIPUS group rate of diarrhea andmortality compared with the control group, were lower than the controlgroup, a statistically significant (p <0.05); CSF group compared with thecontrol group, diarrhea rate was higher, but not statistically significant;mortality rate higher than that of the control group, and was statisticallysignificant (P <0.05).LI PUS group PLT1day after irradiation to normal after stopping theirradiation falls within the normal range, CSF group PLT increased at1dayafter injection, but then decreased to5days injection has been reduced to within the normal range, the control group PLT has fluctuated within thenormal range. Although the the LIPUS group of PLT higher than the CSFgroup and the control group, but among the three groups were notstatistically significant.LIPUS group, RBC and HGB CSF group and the control groupcontinued to decline, LIPUS group compared with the CSF group LIPUSgroup compared with the control group, CSF group and the control group,three groups of RBC and HGB were not statistically significant.3Extend LIPUS irradiation Days, LIPUS group WBC began to rise in1days after irradiation, peaked at7days after irradiation, and then decreasedto the normal range, to40days is still within the normal range.4-27day,LIPUS group compared with the control group, LIPUS group WBC higherthan that of the control group, and was statistically significant (P <0.05).LIPUS group of rabbit model in4days is no longer diarrhea, diarrhea6,no deaths occurred, the diarrhea was20%, mortality was0%. Diarrhea anddeath of the control group there has been of diarrhea26only, diarrhea was86.67%;22deaths only, the mortality rate was73.33%.Diarrhea and deathof the control group there has been LIPUS group compared with the controlgroup, diarrhea and mortality rates were statistically significant, far lowerthan the control group. ConclusionLIPUS increased the WBC Leukopenia Animal Model in New Zealandrabbits by increasing the number of neutrophils, lymphocytes andmonocytes, to improve immunity and resistance to infection, reduce thediarrhea morbidity and mortality, and may also bebeneficial to anti-tumortherapy. |
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