The Exploration Of ELF-MF And Aluminum Damage To CNS And Immune System And The Mechanisms On Mouse

Objective: To investigate the damage and mechanism of ELF-MF andaluminum on CNS and immune system of mouse.Methods: The SPF Kunming mouse accept intragastric administrationof aluminum gluconate (200mg Al3＋/kg)once a day,and6days every weekfor8weeks, and/or expose to ELF-MF(2mT,4h/d). After each aluminumadministration, the exposure was taken in. The load aluminum group alsointragastric administrated GlcNAc. Morris water maze was used to detectthe ability of spatial learning and memory function.Morphologic changes ofcerebral cortex and hippocampus neurons were evaluated by HE staining.The activity of SOD and the content of MDA in brain, spleen and serumwere evaluated by biochemistry. The expression of IL-2, IL-4and INFγmRNA in spleen were detected by RT-PCR. The tau protein, phosphorylatedtau protein and O-GlcNAc protein were detected by Western-blotting.Results: Compared with the control group, the spatial learning andmemory function of ELF-MF group and load aluminum group weresignificantly damaged, and cerebral cortex and hippocampus neurons showed obviously decreased. The SOD activity significantly decreased andthe content of MDA obviously increased in brain, spleen and serum. ThemRNA expression of IL-2, IL-4and INFγ in mouse spleen tissue obviouslyreduced. The expression of phosphorylated tau protein increased while theO-GlcNAc protein decreased both in cerebral cortex and hippocampus.Compared with the load aluminum group, the learning and memoryfunction were improved in GlcNAc group and prevented the neurons lose,and significantly decreased the activity of SOD and blunted the increase ofMDA content can see. The expression of phosphorylated tau proteindecreased while the O-GlcNAc protein increased both in cerebral cortexand hippocampus.Conclusions: ELF-MF and aluminum both injury CNS and immunesystem. The mechanism may involve in oxidative stress, and the damage onbrain also related to the unbalance of phosphorylation and O-GlcNAc ontau protein, the result was emerged over-phosphorylated tau protein.