The Imbalance Of Treg/Th17Cells In Patients With Aplastic Anemia And Regulative Role Of Umbilica Cord-derived MSCs

Objective To explore the role of the balance of Treg/Th17cells inpathogenesis of aplastic anemia （AA）, and to investigate the regulatoryeffect of umbilical cord-derived mesenchymal stem cells（UC-MSCs） onthe ratio of Treg/Th17cells in patients with AA.Methods1、UC-MSCs were isolated, cultured and identified in vitro.2、Flow Cytometry（FCM）was used to detect the percentage of Tregcells and Th17cells among peripheral blood mononuclear cells（PBMCs）from10healthy controls and15AA patients respectively by FCM, and theratio of Treg/Th17was compared.3、PBMCs of the patients with AA was cultured solely and co-culturedwith UC-MSCs for72hours, the percentage of Treg cells and Th17cells inPBMC and the ratio of Treg/Th17were compared respectively between thetwo groups.Results1、Surface antigens of MSCs, CD90、CD105with positive rate≥98% and CD34、CD45positive rate≤1%were detected by the FCM.2、The percentage of Treg cells in AA patients group peripheral bloodwas significantly lower than that in healthy control group(P <0.05), whilethe percentage of Th17cells was higher and the ratio of Treg/Th17cellswas lower.3、Since PBMCs of AA patients was co-cultured with UC-MSCs, thepercentage of Treg cells in AA patients group peripheral blood wassignificantly higher than that in the sole-culture group(P <0.05), while thepercentage of Th17cells was lower, and the ratio of Treg/Th17cells washigher than that in the sole-culture group.Conclusion1、The differential imbalance between Treg cells and Th17cells occursin the peripheral blood of AA patients.2、UC-MSCs may regulate the imbalance between Treg cells and Th17cells by inducing the production and aggregation of Treg, and inhibiting thedifferentiation of Th17.