Effect Of Oxidized Konjac Glucomannan On Blood Lipid And Anti-oxidation Of C57BL/6J Mice Fed With High-fat Diet

The researches of konjac glucomannan (KGM) focused on the physical structure, physicochemical properties and chemical modification. The researches of health function of KGM were less relatively. The study of oxidized konjac glucomannan(OKGM) also focused on the physicochemical properties. The study of health function of OKGM was rarely reported. This article studied on the effect of OKGM on blood lipid and anti-oxidation of mice. The specific research content and results were as flows:(1) The security of OKGM was evaluated by acute toxicity and30d feeding experiments. The results of acute toxicity test showed that, OKGM’s oral LD50was greater than21.5g/kg, according to the standard of acute toxicity dose of food security assessment, OKGM was non-toxic substance. During the30d feeding period, the activity of rats was normal, skin was smooth and color of stool was normal. No obvious symptoms of poisoning and deaths were occurred. There were no significant differences on hematological parameters and biochemical parameters between experimental groups and control group. Through visual inspection, organs’hardness and color of each group was normal. There were no obvious toxic pathological changes on organs morphology in control group and the highest dose group. It could be concluded that, OKGM was no toxic effects on rat organism.(2) It showed that, by determining the weight of body, organ and adipose tissue of mices, OKGM of high-dose(50g/kg) and middle-dose(25g/kg) could reduce the body weight of mice significantly. There was no obvious differences on liver weight between OKGM groups and high-fat modle group. But OKGM reduced the weight of kidney significantly. OKGM also decreased the weight of adipose tissue significantly. Overall, OKGM played a positive role in inhibiting the increase of mice’s body weight and adipose tissue.(3) It showed that, by determining blood lipid(TC, TG, HDL-C, LDL-C), OKGM decreased TC, TG and LDL-C significantly. There was no significantly differences in each groups’HDL-C, so it could be concluded that the high-fat diet did not cause negative impact on HDL-C. (4) The results showed that, by determining anti-oxidation(T-AOC, SOD, GSH-Px, CAT and MDA) of mice, OKGM could improve the activity of T-AOC, SOD, GSH-Px and CAT in serum and liver obviously. It could also reduce the content of MDA in serum and liver significantly.(5) The observation of morphological changes of liver and kidney indicated that, the degree of hepatic steatosis was significantly reduced with the increase in OKGM dose. Less lipid droplets were found in hepatocytes of high-dose and middle-dose group. Hepatocytes cord arranged in neatly, and the structure of hepatic lobule cells tended to be normal. Similarly, with the increase in OKGM dose, swelling of epithelial cells of the renal tubules was improved gradually, and the structure of epithelial cells tended to be normal.