| Guided Bone Regeneration has been widely used in the field of periodontal, planting and oral and maxillofacial surgery. The barrier membrane plays a provital role in the process of protecting the defect from invasion of epithelial and connective tissues, that may otherwise prevent of new bone tissue. In recent years, many researches have been done on GBR, and many scholars have done a lot of work in development, application and other respects of membranes. But most of reseaches mainly focused on the clinical application, and the membranes from other countries which is so expensive that limit their application. In this study,we used polylactic acid, hydroxyapatite, silk fibroin as the raw materials to develop a novel absorbable membrane. And we studied its physical and chemical properties, barrier function and cytocompatibility.Chapter one Preparation and Properties of Polylactic Acid/Nano-hydroxyapa-tite/silk fibroin GBR membranesObjectiveTo prepare and characterize a novel PolylacticAcid/nano-hydroxyapatite/silk fibroin (PLA/n-HA/SF) composite membrane, for purpose of evaluating the feasibility of the composite membrane as the barrier membrane of guided bone regeneration(GBR).MethodsThe PLLA/n-HA/SF composite membrane was prepared by thermally induced phase separation in this research, and was tested on characterization by Scanning Electron Microscopy(SEM) and Fourier transform infrared spectroscopy (FTIR) Furthermore, The porosity of the PLLA/n-HA/SF composite membrane was calculated.ResultsThe composite membrane had three dimensional porous structure with nano-fibrous matrices, and had good uniformity with regular diameter of160-320nm and pore size of1～4μm. The FTIR results show that the experimental group has characteristic peak of the polylactic acid, Nano-hydroxyapatite and silk fibroin, indicating the binding force during the three materials was strong. The composite membranes also had high porosity (>92%).ConclusionsThe PLLA/n-HA/SF composite membranes prepared in this study has favorable structure and exhibits nice porosity.Chapter two Study on the effect of biological barrier of Polylactic Acid/Nano-hydroxyapatite/silk fibroin membranesObjectiveTo investigate the barrier function of PLLA,PLLA/n-HA and PLLA/n-HA/SF composite membranes.MethodsThe the human gingival fibroblasts (HGF) were seeded (seeding density:5×103/ml) on the PLLA,PLLA/n-HA and PLLA/n-HA/SF composite membranes. Scanning electron microscopy was performed at days3to observe the positive and negative sides and the section of the three kinds of membranes.ResultsThe HGF adhered and stretched in positive side of the three kind membrane materials and no cells penetrated into the membranes.ConclusionsThe PLLA/n-HA/SF composite membrane prepared in this study has good barrier function, which meet the basic requirements of guided bone regeneration.Part three Experimental study on cytocompatibility of polylactic acid/nano-hydroxyapatite/silk fibroin membranesObjectiveTo evaluate the cytocompatibility of polylactic acid/nano-hydroxyapatite/silk fibroin membranes, providing the basis of further research for guided bone regeneration membranes.MethodsThe MG63cells were seeded (seeding density:5×103/ml) on the PLLA, PLLA/n-HA and PLLA/n-HA/SF composite membranes. After1h,4h,7h and1d,3d,5d, used CCK-8to measure the cell adhesion and proliferation, and after3days observed the morphology of the cells by scanning electron microscope.ResultsCompared with the pure PLLA membrane and PLLA/n-HA membrane, the PLLA/n-HA/SF composite membrane can improve the cell adhesion and proliferation. Scanning electron microscopy also showed that the MG63cells in PLLA/n-HA/SF composite membrane grown well with fusiform and irregular forms. On the surface, many cell process and pseudopodium closely linked with the membrane.Conclusions Three types of composite membranes especially PLLA/n-HA/SF has good biological properties, which provide the basis of further research for guided bone regeneration membranes. |
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