Incomplete Spinal Cord Injury Rehabilitation-related Research

ObjectiveThe research on recovery of incomplete spinal cord injury, focused on thefollowing issues:①studies of Incomplete spinal cord injury rehabilitationmechanism: explored the feasibility of stem cells to repair spinal cord injury:observed Hes1protein expression levels in the process of neuron-like cells invitro differentiation of muscle-derived stem cells (Muscle-derived stem cellsMDSCs), to study the possible mechanism of differentiation.②Clinical analysisof incomplete spinal cord injury: Retrospective study on the nursing and clinicaltreatment of traumatic incomplete spinal cord injury (TISCI) patients to explorethe impact on the outcomes of traumatic incomplete spinal cord injury patients,and provided a theoretical basis for clinical medical staff.Methods1.Study on the mechanism of recovery: Neonatal rat skeletal muscle afterdigestion was cultured in vitro proliferation of the5th generation after passagecells were divided into two groups: the uninduced group complete mediumculture, the induction group joined inducer culture. Two sets of changes in cellmorphology observed after training6days; the row NSE (neuron-specificenolase neuron-specific enolase) immunocytochemistry and Hes1proteinimmunofluorescence and Western Bloting expression was detected.2.Analysis of clinical rehabilitation: Retrospective analysis of115clinical casesin May2009~June2012admitted in our hospital after the trauma withincomplete spinal cord injury (TISCI).Relevant factors on the recovery effect:Analysis on the general condition, injury status, treatment and nursing situation,effect on the rehabilitation of patients were evaluated by Index brathel;SPSS17.0statistical package was used for descriptive analysis, t-test,chi-square test. ResultsStudy on the mechanism of recovery:1.Induced cells NSE positive staining, non-induced group hardly expressedresult of the immune fluorescent and Western Bloting displayed that The Hes1protein in uninduced group shown strong expression while in the inductiongroup hardly expressed.Analysis of clinical rehabilitation：2.The results show gender distribution in patients with different age differencestatistically significant (P＜0.05), patients with more men than women in all ages,the age concentrates much in40~59years,complications are: respiratory,urinary tract infections and pressure ulcers, various, The percentage are7.8%,6.1%and1.8%.Mainly wounds are fall and traffic injuries, The percentage are39.1%and27.8%; The main damage spot is cervical spine injuries, accountingfor70.4%.The percentage of surgical treatment is68.7%; The percentage ofhauling treatment is54.8%; The percentage of early use of hormone is78.3%.3.Admission and discharge from hospital the brathel index was significantlyDifference (P＜0.05); Among the factors of each age group, the site of injury,cause of injury, the brathel indexes were compared respectively with significantdifference (P＜0.05).Conclusions1.Study on the incomplete spinal cord injury rehabilitation mechanisms: Ratmuscle-derived stem cells in specific microenvironment induced to differentiateinto neuron-like cells and during the differentiation process Hes1proteinexpression reduced.2.Analysis of incomplete spinal injury in clinical rehabilitation：TISCI mainly tocervical injury; with the treatment and nursing care in system, patients have abetter recovery in activities of daily living.