| Objective:Use PLGA (PLA:PGA=85:15) as the wall material of the micro-sphere system, the contents using NGF, NT-3, BDNF, create a diameter of about300um polymer release microspheres implanted damagedsciatic nerve, to prove that the microspheres have a role in the repair of sciatic nerve injury.Methods:Using PLGA material was dissolved in dichloromethane, containing NGF and NT-3, BDNF’s phosphate buffer, by stirring, ultrasonic emulsification, filtered, after the step of centrifugation, etc., made of high-containing neurotrophic factormolecular microspheres. And detection microcapsules in vitro release effect. And then take the30adult SD rats as subjects, divided into experimental and control groups, revealed the sciatic nerve in rats left leg below the piriformis about1cm anastomosis be cut off, the experimental group along the sciatic nerve anastomosis sciatic nerve acts wale implanted polymer microspheres6. Control group, only nerve anastomosis., And each group in the6weeks after surgery side triceps muscle wet weight was measured, electrophysiological testing, tissue sections were observed in each group after6weeks of SD rat sciatic nerve function recovery. The microcapsules were measured again and remove the use of in vitro release effect compared with the previous data.Results:1.We made the microsphere.2.SD rats were implanted microspheres experimental group were killed a control group killed two, not included in the experimental results. The remaining SD rat sciatic nerve function recovery of postoperative experimental group than the control group. The triceps surae determination of experimental rats triceps muscle weight was significantly higher.(Statistical indicators P<0.01), nerve conduction velocity and maximum amplitude of electrophysiological testing in the experimental group was significantly better than the control group. (Statistical indicators P<0.01) tissue sections visible in the experimental group of freshmen number of nerve fibers, more densely arranged in uniform and visible capillaries of newborn and myelin compared to the control group should be thick. The number of nerve fibers and the intensity of the control group were less than the experimental group, and the uneven arrangement, more scattered.3.This microspheres in solution sustainable release of neurotrophic factors,5days before the release of less, the release rate was significantly higher in the future, and peaked around10-15days and to maintain about45days to45days later releasegradually began to decline, but in60days can still be measured solution neurotrophic factor release.4.SD rats remove microspheres in vitro release test again found still sustained release dosage form.Conclusion:1.Use PLGA as a material for microspheres system can contribute to peripheral nerve repair.2.Equipped with NGF, NT-3, BDNF three kinds of neurotrophic factors PLGA microspheres can promote SD rats after sciatic nerve injury repair.3.Microspheres system in vitro release works well, and after use from the animals remove the microspheres still contains a certain nerve growth factor and may continue in vitro sustained release.4.Nerve suture technology, built-in dose of microspheres little difference and the microsphere degradation of mixed can significantly affect the SD rat sciatic nerve injury repair effect. |
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