Identification THRSP As The Interactive Protein Of LOH12CR1

Preface:LOH12CR1, identified on chromosome12, is extremely conservitive in evolution. Its biological function is not clear. Our reaserch group also had screened the Thyroid hormone responsive protein (THRSP, also known as Spot14and S14), as an interactive protein of LOH12CR1. The Spot14gene encodes a protein that is predominantly expressed in lipogenic tissues, which is frequently amplified in breast tumours, and as a result, it has been suggested that this protein could be playing a role in the metabolism and growth of these kinds of tumours.Objective:Our reaserches will prove whether the THRSP actually interacts with LOH12CR1, and then provides a new orientation for the study of LOH12CR1.Methods:Co-transfected HEK293cell with pcDNATM3.1/myc-His-LOH12CR and pCMV-tag2B-THRSP, and then according the methods of co-immunoprecipitation assay and immunofluorescence, we aim to identify the interaction between LOH12CR1and THRSP, and the co-immunolocaliztion of LOH12CR1and THRSP.Results:l.We observed that the THRSP can interact with LOH12CR1by co-immunoprecipitation.2. We observed that the THRSP can co-locate with LOH12CR1by co-immunolocaliztion.Conclutions:Our research has confirmed that LOH12CR1can interact with THRSP. Since the function of THRSP is well accepted as a necessary protein which is involved in fatty acid synthesis and highly expressed in fat-releated tumours, especially breast tumours, we guess LOH12CR1could be playing a role in the fatty acid metabolism and growth of breast tumours which is needed to prove in our further reseach.