| Objective To investigate the expression and distribution of adipocyte fatty-acid binding protein (A-FABP) in the pancreas of Non-obese diabetic (NOD/ShiLtJ) mice.Methods Female NOD/ShiLtJ mice (n=10) and age-matched female BALB/c mice (n=10) were both randomly assigned to two groups:fasting group (fasting for16hours, n=5) and fed group (fed normally, n=5). Therefore, there are four groups (fasting NOD/ShiLtJ group, fasting BALB/c group, fed NOD/ShiLtJ group, fed BALB/c group). All the mice were weighed and the blood glucose were measured. The mice were anesthetized by chloral hydrate and the pancreas were taken out. Part of the pancreas was lysed, of which the total protein level was measured by BCA and A-FABP was measured by ELISA. The expression level of A-FABP was evaluated by the ratio of A-FABP and total protein concentration. The other part of pancreas was fixed in formalin, embedded in paraffin and cut into sections. Immunofluorescence staining was used to detect the expression of insulin and A-FABP.Results1.The level of pancreatic A-FABP in fasting NOD/ShiLtJ group was significantly higher than which in fasting BALB/c group (P <0.001), however, there were no significant differences of pancreatic A-FABP levels between fed NOD/ShiLtJ group and fed BALB/c group.2. The level of A-FABP in the pancreas was significantly higher in the fasting group than which in the fed group in NOD/ShiLtJ mice (P=0.001), however, there were no significance differences between fasting group and fed group in BALB/c mice.3. A-FABP was existed in both insulin-staining β-cells and the periphery of the islet.Conclusions1.The level of A-FABP in the pancreas was significantly higher in NOD/ShiLtJ mice than which in BALB/c mice at the fasting state.2. In the NOD/ShiLtJ mice, the level of A-FABP was significantly decreased after fed compared to fasting state.3. A-FABP was expressed in both insulin-staining β-cells and the periphery of the islet. |
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