Serum According To Traditional Chinese Medicine Treatment Of Acute Cerebral Hemorrhage Rat Model Of Optimal Intervention Time Correlation Of Experimental Research

Purpose:This topic research content belong to the internal medicine ofTraditional Chinese medicine enceohalopathy (direction),the purpose is Throughthe determination of the same blood loss (this experiment all Choose40ul) inthe serum of according to the change of protein content, Discuss the best deliverytime,looking for traditional Chinese medicine Conserwative treatment in theacute phase of ingtracerebral hemorrhage Optimal intervention time, the newbasis for the clinical diagnosis Laboratory data accumulated, lay the foundationfor follow-up study.Methods: using stereotaxic technique, the tail get blood on experimentalCerebral hemorrhage in rats model of preparation, postoperative lavage formedicine, daily record form change of rats, nerve function defect grade andaccording to the weight, take blood serum separation at different time points,beheaded in brain specimens, using ELISA kit for enzyme-linked immune accordingto protein content in serum were determind.Result: After12hours, compared with model group and sham operation group,the serum S100level increased, with statistical significance (P<0.01). Thetreatment group compared with the model group, the serum S100level was lower,with significant statistical significance (P<0.01). After first days, comparedwith model group and sham operation group, the serum S100level increased, withstatistical significance (P<0.001). The treatment group compared with the modelgroup, the serum S100level was lower, with significant statistical significance(P<0.01). After third days, compared with model group and sham operation group,the serum S100level increased, with statistical significance (P<0.001). Thetreatment group compared with the model group, the serum S100level was lower,with significant statistical significance (P<0.01). After fifth days, comparedwith model group and sham operation group, the serum S100level increased, withstatistical significance (P<0.001). The treatment group compared with the modelgroup, the serum level of S100decreased slightly, no statistical significance (P>0.05). Sham operation group, after twelfth hours, first days, third days andfifth days, the serum level of S100change with time was not statisticallysignificant (P>0.05). The model group, after twelfth hours, first days, thirddays and fifth days, the change of serum S100level change over time with astatistically significant (P<0.01). Further analysis showed that, compared withthe twelfth hours, significantly increased the first day, third day and fifthday of the serum level of S100, with significant statistical significance(P<0.01),(P<0.001),(P<0.001). The treatment group, after twelfth hours, firstdays, third days and fifth days, the change of serum S100level change over timewith a statistically significant (P<0.01). Further analysis showed that,compared with the twelfth hours, significantly increased the first day, thirdday and fifth day of the serum level of S100, with significant statisticalsignificance (P<0.05),(P<0.01),(P<0.001). For each time point of S100afterthe drug serum concentration and sham operation group difference can be seen,in the12h,1days,3days and three time points, serum S100difference wasstatistically significant (P<0.05),(P<0.01). In fifth days, serum S100concentration difference was not significant (P>0.05).Conclusion:(1)treated with traditional Chinese medicine couldsignificantly reduce the deficit model of neural function in rats with cerebralhemorrhage; influence the level of serum S100protein of cerebral hemorrhagerats.(2)the best time prompt intervention of traditional Chinese medicine wasadministered for third days.(3)evaluation indicator of the extent of braintissue S100levels in response to changes in cerebral hemorrhage, when the2.94ng/ml to4.37ng/ml range, brain tissue damage is irreversible.