Based On The Antidepressant Effect Of The Chaihu Jia Longgu Muli Decoction(CJLMD) Studies

Chaihu Jia Longgu Muli Decoction (CJLMD) is one of the ancient prescriptions which have effective result on Depression. This article mainly from the formula compatibility research on several aspects, the dose effect relationship, promote the regeneration of neurons, and study its mechanism of treatment of depression.First chapter is theory research, briefly describes the ancient physicians understanding of depression and commonly used representative prescription, radix bupleuri add keel oyster soup formula compatibility principle are analyzed, a preliminary study of radix bupleuri plus keel oyster soup drug dosage and effect relationship. The sides by half quantity small bupleurum decoction to liquorice and keel, oysters, cassia twig, poria cocos, red lead, rhubarb the medicine In small bupleurum soup and solution to Yang, less XuanChang cardinal, trapped in the evil, to pivot turn out; Add cassia twig, not take its muscle functions of solution, and to its da tong Yang, help small bupleurum roll-out in evil; A small amount of rhubarb, is not a good fierce injury are the disadvantages, and there is leakage of heat and power of the stomach; As for keel, oysters, red lead, town of nerves, arresting convulsion stop;Wonderful in poria cocos blindly, can light permeability, water, dredge the sanjiao (triple energizer), and calming nerves nerves to stop surprised; Licorice, not to the gansu slow sex get in the way of evil spirits By the settlement agent of shaoyang, tong Yang heat release, calming nerves of nerves, the original rule "under the typhoid9,8, the chest full of surprised, urination, delirious speech, a heavy, cannot turn side". Is often used for depression and other mental diseases of the nervous system. Is small bupleurum decoction has evolved, but efficacy and attending "mass" of change. Preliminary research shows that radix bupleuri with "dragon negundo bolus of yellow"(small bupleurum decoction to join part of radix bupleuri plus keel is a major component of oyster soup antidepressant.Antidepressant research focus in recent years in the second part, from, promote the regeneration of neurons, using patch clamp technology screening of radix bupleuri add keel oyster soup the antidepressant effect of target ion channels. Results show that neural stem cells by the radix bupleuri add keel oyster soup containing medicine serum after differentiation, potassium channel current density increased significantly, compared with10%FBS group prompt radix bupleuri add keel oyster soup containing medicine serum can promote the increase of potassium ion channel function, and further clarify target ion channels and the induction of neural stem cells differentiationThe third part, discusses radix bupleuri add keel oyster soup antidepressant dose proportion and concentration-response relationship. In mice tail suspension fixed time, serotonin acid induced mice times as flipping their indicators, remove the radix bupleuri add keel oyster soup for single radix bupleuri, radix bupleuri part (radix bupleuri),"yellow dragon negundo bolus" part (keel, oysters, rhubarb, cassia twig, poria cocos), with uniform design experiment method to split radix bupleuri add keel oyster soup research. Results show that the single radix bupleuri, dragon negundo bolus of yellow part can significantly shorten the mice tail suspension time, increase serotonin acid induced mouse head. Simultaneously in a single radix bupleuri dose levels in2.5g/kg, small bupleurum part of4.5g/kg, dragon negundo bolus of yellow part5g/kg, total party of radix bupleuri add keel oyster soup antidepressant effect is strongest. To a certain extent, to clarify radix bupleuri class party because of the different drugs, drug dosage, increase or decrease so that the main function of qualitative change, class party concentration-response relationship for further research has opened up a new train of thought.