Expression And Significance Of H3K9ac And H3K9me3and MVD In Breast Invasive Ductal Carcinoma

objective We sought to investigate the expression of histone H3lysine residues9acetylation (H3K9ac), Microvessel density (MVD), histoneH3lysine residues9trimethylation (H3K9me3) in breast invasive ductalcarcinoma and breast benign adenosis, and to identify the clinical significanceof H3K9ac, H3K9me3, MVD and its relationship among them, so that provideclinical reference for early diagnosis and therapy of breast cancer.Methods: Tocollect sample from the West China Hospital of Sichuan University Surgeryduring March2011to December2011.The breast cancer case which wastaken surgical removal and confirmed invasive ductal carcinoma bypathological and the case with integrity clinical data who have not receivedchemotherapy or radiotherapy before surgery.taken. The number of the casewas73,with an average age of50.70years old,including61cases of invasiveductal carcinoma of the breast. according to the WHO grading standards: level I,grade II25cases, grade III32cases. Lymph node metastasis in37cases,24cases without lymph node metastasis. The benign breast adenosis was12cases.The expression of H3K9ac,H3K9me3and MVD in breast invasive ductalcarcinoma were detected by immunohistochemical SP method. Use the packageSSPSS17.0data analysis, count data chi-square test, t test and F test analysis ofmeasurement data, ordered categorical data using the Spearman rankcorrelation analysis. Significance level α=0.05, p <0.05was considered statistically significant.Results:The expression of H3K9ac,H3K9me3andMVD which was marked by CD34.in breast invasive carcinoma and benignbreast adenosis: H3K9Ac and H3K9me3were mainly expressed in the nucleus,CD34was mainly expressed in vascular endothelial cells.(1) The expression ofH3K9ac was Individually.From the low level to medium level to high level ofexpression rates were39.3%(24/61),44.3%(27/61),16.4%(10/61). In thebenign breast adenosis, the expression rates were0,25%(3/12),75%(9/12).The difference between the two statistically significant (x2=18.859p<0.05).There was relationship between H3K9ac with lymph node metastasis (p<0.05),and H3K9ac was no relationship with WHO histological classificationthe estrogen/progesterone receptor (ER/PR).(p>0.05).(2) The expression ofH3K9me3was Individually.From the low level to medium level to high lever ofexpression rates were6.6%(4/61),59.0%(36/61),34.4%(21/61). In benignbreast adenosis organization, H3K9me3low expression in41.7%(5/12),moderate expression rate of8.3%(1/12), highly expressed in50%(6/12). Thedifference between the two was statistically significant.(x2=15.75, p <0.05)The positive expression of H3K9me3was regardless with breast cancerWHO histological grade, lymph node metastasis, and the estrogen/progesterone receptor (ER/PR)(p>0.05).(3)The number of CD34breast ininvasive ductal carcinoma and benign breast adenosis were34.17±6.27and23.28±6.87. The difference between the two was statistically significant (t=5.411p <0.05). There was relationship between CD34with lymph nodemetastasis (p <0.05) and CD34was no relationship with WHO histological classification the estrogen/progesterone receptor (ER/PR)(p>0.05).(4)Theexpression of H3K9ac in breast cancer tissue from the low level to mediumlevel to high level of expression,The values of MVD were37.24±5.71,33.13±5.95,29.60±5.03. The difference between the the expression changes H3K9Acwith breast cancer the MVD has a statistically significant (F=7.097, p<0.05).The relationship between H3K9Ac and MVD was negatively correlated(rs=-0.441p <0.05). H3K9me3low, medium, high expression in breastcancer tissue,the values of MVD were27.15±4.47,33.93±6.70,35.90±4.85.The changes of H3K9me3expression with the difference MVD of breastcancer tissue was statistically significant (F=3.620, p <0.05). H3K9me3andMVD was positively correlated (rs=0.3, p <0.05). In low expression ofH3K9ac, H3K9me3low, medium, and high expression of sequentially were1,11,12; of moderate H3K9Ac expression in breast cancer cases in H3K9me3low, medium, and high expression were3,15,9; the highly expressed H3K9Acbreast cancer cases in H3K9me3low, medium and high expression0,10,0.H3K9ac and H3K9me3was negatively correlated, there was significantdifference between the two.(rs=-0.303p <0.05).Conclusion:(1)The expressionof H3K9ac in invasive ductal carcinoma was less than benign breastadenosis.There was relationship between H3K9ac with lymph node metastasis,H3K9ac was no relationship with WHO histological classification the estrogenrecepto（rER） andprogesterone receptor (PR).(2) The expression of H3K9me3in invasive ductal carcinoma was higher than benign breast adenosis. Thepositive expression of H3K9me3was regardless with breast cancer WHO histological grade, lymph node metastasis, and t estrogen receptor （ER）andprogesterone receptor (PR).(3) The expression of CD34in invasive ductalcarcinoma was higher than benign breast adenosis. There was relationshipbetween CD34with lymph node metastasis and CD34was no relationship withWHO histological classification estrogen receptor （ER） andprogesteronereceptor (PR).(4)The expression of H3K9ac is negatively related to theexpression of P27in breast cancer.H3K9ac and H3K9me3may lead to thebreast cancer by change histone modification.(5)Regulate the expression ofH3K9ac and H3K9me3,control the gene transcriptional,may provide a newstrategy and the basis to the treatment of breast cancer.