Expression Of Growth Factor Receptor Bound Protein7in The Endometrium Of Early Pregnant Mouse

ObjectiveTo explore the role of growth factor receptor bound protein7(Grb7)during embryo implantation through detecting its expression pattern in themouse endometria.MethodsSpecimen collection: Established Kunming mouse model ofnon-pregnant and pregnancy d1to d7days and separation these endometrialtissue,then immediately frozen at-80℃for further test.Performed Real Time-PCR, immunohistochemistry and Western blot toexamine the expression level of Grb7mRNA and protein in the non-pregnantand pregnant mouse endometria from d1to d7.Function test: Grb7antisense oligonucleotides was injected at2000h onpregnant d3, and examined embryo implantation number on pregnant d7.ResultsGrb7mRNA expressed in uterine endometrium of the non-pregnant andpregnant mouse from d1to d7, and reached to the highest level on pregnant d5by Real Time-PCR. Immunohistochemistry and Western blot showed thatthe expression pattern of GRB7in mouse endometria was the same with thatthrough Real Time-PCR.Moreover, from pregnant d1to d4, GRB7was mainly expressed inepithelium and glandular cells, and lowly expressed in stromal cells; on d5and d6, Grb7was more intense in epithelium and glandular cells, and startedto express obviously in stromal cells, especially on d5.On d6, GRB7expressed strongly in primary decidual zones atimplantation sites and stromal cells at inter-implantation sites, respectively.After horn injecting Grb7antisense oligonucleotides, the number ofembryo implantation was obviously decreased when compared to that in thecontrol group.ConclusionsThe differential expression of Grb7in pregnant mouse endometriasuggested that Grb7may involve in the process of embryo implantation.