Experimental Research About Astragalosi-des’ Influence On IL-1β And TNF-α Level In Model Rat Intervertebral Disc Tissue Of Cervical Spondylotic Myelopathy

Objective: By establishing the rat models of Cervical spondylotic myelopathy(CSM)treated with different dose Astragalosides to observe the influence of the content of IL-1β andTNF-α on the neural function of CSM, discussed the root of re membranous milk vetch totalglucoside inhibit inflammation, thus promoting the mechanism of action of neural functionalrecovery.Methods: Selected8-10week-old SD rats of SPF110, except the18normal rats ofGroup A set as the blank control group, the other rats will be used to establish rat model in theway of planting morphogenesis protein (BMP) in the rear of the cervical intervertebral disc.After model establishment, to remove the dead and the excluded,90model rats will berandomly divided into five groups i.e B, C, D, E and F according to the weight. Each groupcontains18rats, which is respectively model group, high dose Astragalosides group, middledose and low-dose group, the positive control group Mecobalamin. As for Astragalosides, itwill be treated with high dose, medium and low dose, according to the120mg/kg/d,60mg/kg/d,30mg/kg/d standard respectively; while as for Mecobalamin, it will be dosedaccording to the standard of0.05mg per100g body weight. Normal group, model group willbe received an equal volume of saline treatment with4w. Before the death of the rats, inclinedplate test will be proved to monitor the moving function prior to delivery, and1,2,4weeksafter the treatment. After treating1,2and4weeks, the rats were killed for three timesrespectively, the levels of disc tissue cytokines (IL-1β and TNF-α) of spinal cord tissue will bedetected with the analysis of SPSS17.0. The removed spinal cord tissue will be treated by HEstaining to observe the pathological changes in spinal cord tissue.Results:1.The comparison of inclined plate degree: Compared with group A, beforedosing, group B, C, D, E and F are significantly decreased (P<0.05), which showed the modelis successful. Compared with group F, after1week of the treatment, the group D is increased,with statistical significance (P<0.05); Group C and E had reduced with no statisticalsignificance (P>0.05); After2weeks of the treatment, group C and E are significantlyreduced, with statistical significance (P<0.05); Group D is increased, but no statisticalsignificance (P>0.05); After4weeks of the treatment, Group C had reduced, group D had increased with statistical significance (P<0.05); Group E had reduced with statisticalsignificance (P<0.05). Within each group, compared with those before dosing, after4weeksof the treatment, except group A, where there was no statistically significant difference(P>0.05), the other groups differences are statistically significant(P<0.05).2.Comparison of IL-1β: Compared with group B, after1,2and4weeks of the treatment,group C, D, E, F are significantly decreased with statistical significance(P<0.05). Comparedwith group F, group C is increased, group D is decreased with no statistical significance(P>0.05); After4weeks of the treatment, group E is increased with statistical significance(P<0.05), while the other times are with no statistical significance (P>0.05). Each group indifferent periods, group C, D and F after4weeks dosing compared with1week of dosing, thedifference is statistically significant (P<0.05); The other groups are with no statisticalsignificance (P>0.05).3.Comparison of TNF-α: Compared with group B, after1,2and4weeks of treatment,group C, D, E and F are significantly decreased with statistical significance (P<0.05).Compared with group F, group C and E are significantly increased with statistical significance(P<0.05); Group D is decreased with no statistical significance (P>0.05). Within each group,compared with1week after the treatment, except there are no statistically significantdifference in group A (P>0.05), the others are statistically significant (P<0.05) after4weeks.4.Morphological changes in spinal cord tissue: Compared with group B, group C,D, E, F are improved to some extent after1,2,4weeks of treatment. Tissue edemain spinal cord lessened, cavity structure recovered, gray matter neurons swelling improved, the around cell gap decreased. As for the overall recovery, the effect of groupD is close to group F, and was superior to group C and E.Conclusions:1.The establishing of rat model of CSM is successful in the way to implantBMP in the rear of the cervical intervertebral disc.2.Astragalosides can significantly reduce IL-1β, TNF-α level in the intervertebral disc tissue of CSM model, and promote the recovery of neurological function, which can reduce their secretion of inflammatory cytokines, inhibition of the disc inflammation, and relieve the spinal cord oppression and stimulation.3.The research found that there was dose-effect relationship in Astragalosides’ anti-inflammatory response of CSM model in rats intervertebral disc. The function ofAstragalosides in medium dose group is equal to that of contrasting group of Mecobalamin,which is obviously better than the high and low dose group of Astragalosides.