The Effect Of Local Application Of Membrane Fusogen On Degeneration And Regeneration Of Distal Nerve After Repair Of Nerve Injury

Purpose：To observe the effect of local application of membrane fusogen on degenerationand regeneration of distal nerve after repair of nerve injury.Method:56SD rats were randomly devided into the control group and the experimentalgroup,the right sciatic nerves of the both groups were transectedand to establish the sciaticnerve injury model,then the cut ends were sewed up with10-0microscope suture under themicroscope. The anastomotic stomas of the experimental group were treated with30mmol/L PEG,which was applied to the lesion site via a micropipette so that thedenser PEG-containing solution fowed for2minutes over the lesion sites, and thenphysiological saline was employed to wash off the PEG. The anastomotic stomas of thecontrol group were bathed in the physiological saline for about2minutes afterrepiar.Immunohistochemical staining of ED1was performed at3、7、10days postoperation to observe the complete myelin sheath area and to count the number of activatedmacrophages per cross sectional area. Electrophysiological examination and toluidine bluestaining were conducted12weeks postoperation..All results were statistically analyzed.Results: The number of activated macrophages per cross sectional area was more than thatof the control group at all timepoints that the specimens were taken. Electrophysiologicalexamination at12weeks postoperation revealed that nerve conduction velocity of theexperimental group was faster than the control group;axonal number of the experimental group was more than the control group at12-week-point.Conclusion： Local application of membrane fusogen after repair of nerve injury canrelieve Wallerian degeneration to some extent and increase the axonal number ofregeneration.