| Objective：Arthritis is one of four main diseases that can cause patients disability. Thepatients not only endure joint pain and inconvenience brought by joint dysfunction,but also they need to bear enormous economic burden. These seriously affect patients’life quality. Hyaluronic acid (HA) is an important component of synovial fluid.The latest research according to the domestic and foreign, from the followingthree aspects of oral hyaluronic acid for the treatment of arthritis:(1)Analysis physical and chemical properties of the HA samples(2)From animal level, observe the effect of HA by oral administration on mouse withadjuvant arthritis(3)From human feeding level, observe the effect of HA by oral administration onpatients with arthritis.Methods：According to the above study, research method as follows:(1)According to the national regulations, detect the pH of the HA sample, heavymetals (arsenic, mercury, cadmium and lead), glucuronic acid, moisture content, fatcontent, Residue on ignition content and characteristics of viscosity detection, whileuse viscosity detection to calculate molecular weight of HA sample.(2)408-week-old healthy mice were divided into4groups: normal，model，HA anddiclofenac group. Mice in normal group were injected0.1mL normal saline into theright rear foot to establish animal model. Other groups were injected0.1mLFluoride’s complete adjuvant (FCA) to establish animal models with adjuvant arthritis.Mice were treated after inflammation second day. The mice in normal group andmodel group were given normal saline, HA group were given HA sample (200mg/kg)and diclofenac group were given diclofenac sodium (0.1mg/kg). All mice had begiven once a day for28days. After28days, the weight, thickness of foot metatarsusand swelling of mice knee were observed while concentration of HA and interleukin-1(IL-1) in serum was tested by enzymelinked immunosorbent assay (ELISA).(3)Select10patients with knee pain (53.70±7.79years) as subjects. They had eatenHA capsule for8weeks. We had followed up patients for8weeks. Efficacy wasjudged by Kellgren-Lawrence grading and WOMAC. Judge the safety of oral HA bycontrast blood tests, which were before and after oral HA.Results： (1)Average pH value of HA sample was6.1. The heavy metal content in the statewithin a limited range of national standard. The test showed the content of As in HAsample was Glucuronic acid average content was47.870%. Average moisture contentwas6.279%. Average crude fat content was0.067%. Average residue on ignitioncontent was17.858%. Average intrinsic viscosity was1599.960cm3/g, Averagemolecular weight was907kd.(3)After treatment, compared with the model group, thickness of foot metatarsus andarthritis index reduced significantly (P <0.05), IL-1and HA content in serum werelower than the model group (P <0.05). And there was no significant differencebetween HA group and diclofenac group.(3)Compared to the score before oral HA, the WOMAC scores were significantlyreduced. WOMAC score standards include three projects that are pain, stiffness andjoint function. Patients gave a score according their symptom. The result showed painscores decreased significantly and its decreased rate is29.33%.Scores decreased ratesof stiffness and joint function were19.87%,26.78%, respectively. It found WOMACscores rose slightly, but compared to scores before feeding, there is still a significantdrop. At the same time, blood indexes basically unchanged before and afterexperiment. Kellgren-Lawrence classification did not change before and afterexperiment and joint pathological changes was not yet clear.Conclusions：(1)HA used in experiment met national and industry requirements.(2)Oral hyaluronic acid could effectively alleviate the knee swelling of mice, reduceHA and IL-1content in serum and enhance the activity, which had a good therapeuticeffect on mice with adjuvant arthritis.(3)Oral hyaluronic acid could effectively alleviate patients’ pain, stiffness andimprove joint function. Oral hyaluronic acid was safety and efficacy.Kellgren-Lawrence classification did not change clear. |
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