Pharmacognostic Studies On Caulis Et Radix Solani Capsicoidis

Caulis et Radix Solani Capsicoidis is the dried root and stem of Solanumcapsicoides All. from Solanaceae family recorded in the“Gangdong Province ChineseMateria Medica Standards”. It has effects of dissipating blood stasis, relieving pains,relieving cough and asthma which is commonly used in cold type stomach pain, lungcold type cough, swelling and pain from traumatic, sore carbuncle swollen poison. Itis contained in Guangdong famous“Shaxi herbal tea” for curing seasons cold, whichalso named Ciqie, Dianqie, Dingqie, Hongguo dingqie in Chinese。Due to its reducingresource, there is substitute for medicinal use on the market. Our project intends tobuild the quality standards of Caulis et Radix Solani capsicoidis and also the methodsof identifying it from its substitute.According to plant morphology, the substitute was identified as Solanumaculeatissimum Jacq. Then the two plants were studied in medicinal properties,transverse section, dissociated tissue and temporary section.The main differences arethat the pith of stem in S.aculeatissimum is smaller than S.capsicoides, thatS.aculeatissimum has hooked spine basally flattened in stem and stellate hair in bladeback, but not S.capsicoides, that S.aculeatissimum has more phloem fiber in stemsthan S.capsicoides.Three mobile phase were selected for TCL assay, with chlorogenic acid as thecontrol, polyamide film as stationary phase. Results show that TLC spots of the twoare extremely similar, and both have the same spot with the control. Using the methodof ultraviolet spectral line to further identify the two plants. But due to they are withsimilar chemical composition, this method did not reach the expected purpose ofdiscrimination.In order to further distinguish S.capsicoides and its substitute, HPLC fingerprintmethod was choosed. The chromatographic condition is C18column, gradient elutewith mobile phase of A(Acetonitrile)-B(0.1%phosphoric acid water). The proportion of A phase is10%~18%in0~15min,18%~22%in15~30min,22%~40%in30~50min,40%~80%in50~55min,80%~10%in55~60min.Methodology of the experiment included precision, repeatability and stability wasalso studied. Ten batches of each sample in different regions were determined andreference spectrum was established. According to similarity calculation,S.aculeatissimum has13common peaks, S.capsicoieds10common peaks,8of whichare overlapped. Through the similarity evaluation, matching degree of S.capsicoiedsand S.aculeatissimum is from0.37to0.75%, this method can effectively distinguishthese two herbs. Fingerprint results can also be used for the quality control ofS.capsicoiedse.The existence of chlorogenic acid in S.capsicoieds is confirmed by methods ofTLC, UV and HPLC. Therefore the quantitative control of S.capsicoieds wasestablished by HPLC using chlorogenic acid as control. The detection condition isusing C18column, with mobile phase acetonitrile-0.2%phosphoric acid water(13:87),λ=325nm.In order to ensure material supply, cultivation experiment of S.capsicoieds wasexplored. The results show that with efficient water and fertility of soil, S.capsicoiedscan grow well. And the plant morphology of collected seed from two origina isslightly different, their production is also different.