High Performance Capillary Electrophoresis Analysis Of Active Ingredients In Swertia Mussotii Franch., Lamiophlomis Rotate Kudo And Ziziphus Jujube Mill

As the research object, high performance capillary electrophoresis technology was used,determination of several kinds of active ingredients in Swertia mussotii Franch,Lamiophlomis Rotate (L.R.), Cistanche and jujube. Also for other quality standard ofmedicine research laid a certain foundation. The main contents of this dissertation include：1This paper introduced the development of the CE technology, the basic principle, theinstrument structure, the separation mode, the injection techniques, the detection method andhyphenated modes. The application of CE in the the northwest Tibetan medicine wassummarized. The main content and significance of this paper were introduced, too.2The highly performance capillary electrophoresis (HPCE) method was developed forthe simultaneous determination of Gentiopicroside and Swertiamarine in Swertia mussotiiFranch. The effects of some important factors such as pH value, concentration of runningbuffer, separation voltage and detection wavelength, were investigated. Under the optimalelectrophoresis conditions, gentiopicroside and swertiamarine can be well separated anddetected fast. The linear range of Gentiopicroside and Swertiamarine were9.375-150μg/mL，6.252-100μg/mL with correlation coefficients of0.9997and0.9998. The averagerecoveries of gentiopicroside and swertiamarine in sample were ranged from99.74%and99.58%, the R. S. Ds of retention times were6.7%and5.1%, and R. S. Ds of peak areas were1.98%and2.43%. The result showed that the method was simple, convenient, rapid,reproducibility, and can be successfully applied in the determination of Gentiopicroside andSwertiamarine as pharmaceutical ingredients.3In this paper, capillary zone electrophoresis (CZE) with direct UV detection wasdeveloped for the determination of oleanolic acid, ursolic acid, quercetin and apigenin andthen for the first time successfully applied to the analysis of the four analytes in Swertiamussotii Franch and its preparations. Different factors affecting the CZE procedure wereinvestigated and optimized, and the optimal conditions were:50mmol/L borate-phosphatebuffer (pH9.5) with5.0mmol/L β-CD,15kV separation voltage,20°C column temperature,250nm detection wavelength, and5s electrokinetic injection time (voltage20psi). Under the conditions, oleanolic acid, ursolic acid, quercetin and apigenin could be determined within thetest ranges with a good correlation coefficient (r2>0.9991). The limit of detection (LOD) forconditions, oleanolic acid, ursolic acid, quercetin and apigenin was0.3415,0.2003,0.0062,0.2538μg/mL, respectively, and the intra-and inter-day relative standard deviations were nomore than3.79%. This procedure afforded a convenient, sensitive, accurate method forsimultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in S.mussotii Franch.4Despite the separation efficiency of capillary electrophoresis (CE) is much higher thanother chromatographic methods, it is sometimes difficult to adequately separate the complexingredients in biological samples. In this paper, one effective and simple way to develop theseparation efficiency in CE is to add some modifiers in the running buffer. The suitablerunning buffer modifier β-cyclodextrin (β-CD) was explored to fast and completely separatefour phenylethanoid glycosides aglycones (homovanillyl alcohol, hydroxytyrosol,3,4-dimethoxycinnamic acid and caffeic acid) in Lamiophlomis Rotate (L.R.) and Cistancheby capillary zone electrophoresis with UV detection. It was found that when β-CD was usedas running buffer modifier, a baseline separation of the four analytes could be accomplishedin less than20min and the detection limits were as low as10-3mg/L. Other factors affectingthe CE separation, such as working potential, pH value and ionic strength of running buffer,separation voltage and sample injection time were extensively investigated. Under theoptimum conditions, a successful practical application on the determination of L.R. andCistanche samples confirmed the validity and practicability of this method.5High performance capillary electrophoresis (CE) was developed for the determinationof cyclic adenosine phosphate, apigenin and quercetin in jujube. The effects of several factorssuch as concentration of running buffer and the pH, concentration of the additiveβ-cyclodextrin, the detection wavelength and the separation voltage for separating result wereinvestigated. In optimum condition, three groups chemical component have been achievedseparation detection. A good linearity between peak area ratio of the common peak to theconcentration was found in the range of16.5~529.4mg/L for cyclic adenosine phosphate, 5.5~176.4mg/L for apigenin and8.8~282.4mg/L for quercetin, and correlation coefficients (r)are0.9998,0.9998,0.9997, respectively. The average recoveries were97.31%,95.62%and94.88%for cyclic adenosine phosphate, apigenin and quercetin, respectively. The retentiontime of relative standard deviation was6.7%,5.1%and5.3%and the peak area of relativestandard deviation was4.5%,5.8%and4.2%. The results showed that the method was simple,rapid and with satisfactory recoveries and good reproducibilities. It can be used to detection ofelements of cyclic adenosine phosphate, apigenin and quercetin.