Effect Of Gut Parasympathetic Nervous Dues To Pathological Physiological Changes Of Acute Necrotizing Pancreatitis

Objective: To investigate the influence exerted by greater splanchnic nervetransaction on the pathophysiological process of acute necrotizing pancreatitis (ANP)and explore the new method to cure ANP.Methods: Twenty healthy mongrel dogs randomly into sham operation group (n=4), ANP model group (model group, n=8) and ANP model with bilateral greatersplanchnic nerve blockage group (experimental group, n=8). After surgery, the serumlevels of pancreatic amylase (AMY), calcium, high-sensitivity C-reactive protein,tumor necrosis factor-α and interleukin-10were monitored.and at postoperative day7,all the animals were sacrificed and the pathological examinations were performed.Results:1.As for the serum AMY level,group sham operation was significantlylower than model and experimental group, between model、experimental group andsham operation group, there were quite Statistic differences(P＜0.01),there were notStatistic differences (P>0.05)between mode and experimental group.2.As for serumsuper sensitive c-reactive protein level of sham operation、model、experimentalgroups of model prepared success were increased then before, sham operation grouphigher level was low and the falling fast; model Group higher level was highest, fallslowest; experimental group higher level was high level of raised fall slower. betweenmodel、 experimental group and sham operation group, there were quite Statisticdifferences(P<0.01),there were Statistic differences (P<0.05)between model andexperimental group.3. As for serum super calcium levels of sham operation、model、 experimental groups of model copied success were reduced then theprevious, serum super calcium level of sham operation group was not obvious thenbefore, serum super calcium level of group B is lower than before, serum supercalcium level of model Group was lowest then before, between model、 experimentalgroup and sham operation group, there were quite Statistic differences(P<0.01),there were Statistic differences (P<0.05)between model and C experimental group.4. As forserum Tumor necrosis factor alpha levels of sham operation、model、experimentalgroups of model copied success were increased then before and rise to the peak at1day after surgery. sham operation Group elevates level comparatively low and comesdown quickly after peak value; model Group elevates level was most highly,andcome down after peak value speed was the slowest; experimental group elevates levelmedium, come down after peak value; between model、experimental group and shamoperation group, there were quite Statistic differences(P<0.01),there were Statisticdifferences (P<0.05) between model and experimental group.5. As for seruminterleukin-10level of sham operation、model、experimental groups of modelcopied success were increased then before and rise to the peak at24hours aftersurgery, sham operation Group of elevated level was low and falling fast after peak;model group higher level was high and the falling slowly after peak; experimentalgroup increases level was highest and slowest decline after peak. between model、experimental group and sham operation group, there were quite Statisticdifferences(P<0.01),there were Statistic differences (P<0.05)between model andexperimental group.6. As for pathological scores, it’s more serious in model andexperimental groups than in sham operation group and it’s more serious in modelgroup than in experimental group and there are Statistic differences (p<0.05).Conclusion1.Greater splanchnic nerve transaction can reduce severe stress andexcessive inflammatory response of ANP dogs in the early stag.2.Greater splanchnicnerve transaction can alleviate development of pathological process in ANP dogs andhas therapeutical effect on the treatment of ANP dogs.