The Relationship Of MiR-10b Expression Differences And Clinicopathological Characteristics And Prognosis Of Breast Cancer

ObjectiveTo analyse the relationship between the expression of miR-10in human breast cancer，para-carcinoma, benign tumor organization, and the clinicopathologic parameters of breastcancer. In addition, our study aims to investigate its significance in the occurrence andprogress of breast cancer. Furthermore, Return materials of the patients with breast cancerwere collected to analyze the relationship between the levels of miR-10and the prognosisof breast cancer.Materials and methodsThe cases of breast cancer were collected after the resection of breast cancer in ourhospital from January2005to December2007. Among one hundred and seventeen cases ofbreast cancer, ninety-six who had intact data of follow-up are analysed the relationshipsbetween the clinicopathologic features and the prognosis of breast cancer. Then thirty-fivebreast cancer （including twenty metastatic breast cancer organizations and fifteennon-metastatic breast cancer organization）, sixteen relevant para-carcinoma tissues, and fifteen breast benign tumor tissues were selected and embeded in paraffin. Total RNA wasextracted from tissues. Using real-time quantitative PCR, the expression of miR-10b wasevaluated. According clinicopathologic parameters of different tissues,(such as age,histological grade, lymph node metastasis，clinical grade, ER-receptor, PR-receptor,c-erB-2)were divided into different groups. The relationship of the expression of miR-10b with theirclinicopathologic parameters in different groups was analyzed and analyse miR-10bexpression in the occurrence and progress of breast cancer. By breast cancer patient’sretention dates, to analyze the relative expression value of miR-10is relevant to theprognosis of breast cancer.Results1、96cases of breast cancer data show that the age range23to90years old, mean age48.28±14.257, the peak age segment40-60years of age; tumor maximum diameter of11.5cm, with an average size of3.571±2.187; breast cancer after3-year survival was46.2%;mean survival time of42.75,95%confidence interval (40.5,45); median survival time was41.00,95%confidence interval (35.596,42.404).2、The expression of miR-10b in breast cancer tissue cancer and adjacent breast tissue,benign breast tumor tissue was investigated. In the breast cancer group, the levels ofmiR-l0b average expression was higher than the other two groups expression (P<0.05)，and comparison of35cases of breast cancer tissue,20cases of breast metastatic cancergroup and15cases of breast cancer metastasis, two groups of miR-10b expression weresignificant difference (P <0.05).Relative to the adjacent breast tissues, miR-10b expressionin breast cancer were divided into two groups, namely, high expression group (18cases)and low expression group (17cases). Analyzing the difference of two sets of clinical andpathological parameters found that high expression of miR-10b in histological grade, lymphnode metastasis and the C-erbB-2was significantly higher than that of miR-10b lowexpression group (P<0.05). However, there was no significant difference between the twogroup in age、tumor size、ER、PR (P>0.05). Cox regression analysis showed that miR-10bhigh expression is one of the poor prognosis of breast cancer index (P <0.05, HR=4.494).In addition, age、clinical stage、lymph node metastasis is also an independent prognostic factors of breast cancer (P <0.05).Conclusion:The present study demonstrates that there is a close relationship between the levels ofmiR-10b and the pathogenesis of breast cancer and there is also a certain relationship withmiR-10b and the breast cancer metastasis. Our data study suggests that miR-10b would bean effective clinical parameter to indicate the malignance and prognosis of breast cancer.