Cytological Behavior Of Osteoblasts On The TiO₂Nanorods Film Titanium Plate

Titanium and its alloy have a wide range of potential applications in the area of medicine for its stable chemical properties, rich sources and good biomechanical properties. However, as a surgery implant, the synostosis between them and the bone was mechanical but not chemical. Simultaneously, titanium was easy to release ion to body because corrosion in physiological environment. All of these limited the clinical achievement ratio and widespread development of implant technique. Through different surface treatment technique,the implant surface morphology and structure can be improved and the surface bioactivity enhanced,which formed to promote the osseointegration. The biological activity of osteoblasts, the surface characteristics of implant (mainly the chemical composition, surface roughness and morphology, etc.) and the stress environment all may affect the implant osseointegration. Our group ues a new low-temperature oxidation of Ti metal surface modification with hydrogen peroxide (H2O2) to optimize the titanium surface activation. This method enables the metal surface to form a layer of nano-structured titanium dioxide (TiO2) nanorods film, which can strengthen the inner plant contact with the surrounding bone tissue both in contacting area and contact type.what’s more, it may enhance the biological activity of osteoblasts and promote the osseointegration. In this study, on the basis of the development of new titanium metal surface treatment,we also have a deep research of the cellular compatibility. Osteoblast is the most important cells for the bone tissue formation around the implant and its growth activity and biological properties is essential for the formation of bone tissue and bone integration.In the vitro study, the primary cultured osteoblasts of neonatal rat calvaria were cultured on the new titanium surface modified materials. Then the adhesion, proliferation,differentiation and mineralization of osteoblasts were evaluated by cells counting, scanning electron micrography, standard CCK-8assay, activity of ALP and the osteogenesis marker gene detection. Through the research, the biocompatibility of the osteoblast on the TiO2nanorods film titanium plate was studied and we als use an ordinary acid-treated titanium sheet as comparison(control group). The experiment results obtained are described as follows:1The microscopic morphology of new surface treatment titanium plate was different from the ordinary acid-treated titanium plate, its surface had more clusters of nanorods while ordinary acid-treated titanium plate was relatively flat;2Titanium sheet processing by the new surface treatment had no adverse effects on osteoblast cells in early adhesion, proliferation, ALP expression and osteogenic marker gene expression;3The new surfactant treated titanium plate group was conducive to the early attachment of cells, the cell proliferation rate was higher in the early6days. The more active ALP could be observed in the osteoblasts cultured on the nanophase compared to the control group.In a way,the expression of ALP and some of osteogenic differentiation marker genes was promoted;These results indicates that the new surface treatment of titanium sheet had no adverse effects on osteoblasts biological behavior but had a good biocompatibility and enhanced the cell function to a certain extent.This research also provided relevant theoretical and experimental basis for the further animal experiments.