| Objective: To observe neurotrophin-3(NT-3) and neural stem cells (NSCs)transplantation for the treatment of neonatal rats after hypoxia ischemia brain damage(HIBD) in neonatal rat neural electrophysiological manifestations, biopsy and Western blotanalysis. Explore the neurotrophin-3(NT-3) with neural stem cells (NSCs) Combinedtransplantation in the treatment of neonatal rat hypoxic-ischemic brain damage (HIBD) nerverepair.Methods: Totally107-day-old Wistar neonatal rats were randomly assigned to thecontrol group,507-day-old Wistar neonatal rats were selected to establish HIBD animalmodels, and then randomly assigned to the stem cell transplant group, NT-3joint neural stemcell transplantation group, brain injury group. Neural stem cells were transplanted in theinjury side of the lateral ventricle after3days, and in NT-3group, neural stem cells combinedwith NT-3were transplanted, in neural stem cells group, only neural stem cells weretransplanted, in brain injury group, PBS transplanted. After transplanted for three weeks,seven weeks, the nerve electrophysiological was tested, then taking the brain tissue for HEstaining and Western blot analysis.Results: Neurophysiological testing: after3weeks for transplantation, compound actionpotential (CMAP) in rat hind limb quadriceps of rats with NT-3combined with nerve stemcell transplantation was significantly higher volatility, shorter incubation period comparedwith pure stem cell transplantation and HIBD group(P <0.05); after7weeks fortransplantation, CMAP in rat hind limb quadriceps of rats with NT-3combined with nervestem cells transplantation was significantly higher volatility compared with stem celltransplantation and HIBD group(P<0.05), latency is not obvious; HE staining transplant3weeks: In brain injury group, damage lateral ventricle adjacent normal tissue structuraldamaged, there were a number of different sizes of the cysts, in the neural stem celltransplantation group, periventricular normal organizational structure had been restored, andhad rare cystic; in NT-3joint neural stem cell transplantation group, the ipsilateralperiventricular tissue structure recovered more better. HE staining transplant7weeks: brain injury group damage lateral ventricle adjacent normal tissue structure damage, there are anumber of different sizes of cysts, neural stem cell transplantation group and NT-3combinedwith nerve stem cell transplantation group periventricular normal organizational structure hasbeen restoredcystic rare, the ipsilateral periventricular organizational structure recovery is notvery different. Western blot analysis of transplant3weeks, the amount of brain tissue ofGFAP expression was significantly NT-3group than in the group of brain injury, neural stemcell transplantation group expression less, but the amount of expression than the normal group.MBP protein expression in brain tissue is significant, NT-3group more than in the normalgroup, the brain injury group, and the expression levels of neural stem cell transplantationgroup. Transplant7weeks of GFAP expression in brain tissue was significantly NT-3groupthan in the group of brain injury, the expression of neural stem cell transplantation group less,but the amount of expression than the normal group. MBP protein expression in the braintissue of NT-3group was significantly more than the amount of brain injury group expression,the expression levels of neural stem cell transplantation group, but the amount of expressionthan the normal group.Conclusions: Neurotrophin-3(NT-3) with neural stem cells (NSCs) co-transplantation inthe treatment of neonatal rat hypoxic-ischemic brain damage (HIBD) has significant effect. |
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