| Lipid rafts are membrane microdomains that can stabilize to form larger platforms to enhance signalling transduction. Integrins are located on the cell membrane acting as cell surface adhesion receptor molecules. Within the cells are also a number of non-receptor tyrosine kinases, example c-SRC that helps in sending signals from the cell to membrane receptor proteins. Furthermore, these tyrosine kinases also receive signals from membrane receptor proteins into the cell. This form of outside-in and inside-out signal transduction regulates various physiological processes including cell migration, survival, cell-cell adhesion, cell proliferation, angiogenesis and invasion.Cancer cells have been able to adopt these normal physiological processes to their own advantage, thus enabling them to grow and disseminate to distant sites. Using wound healing assay, Transwell migration assay and specific small interfering ribonucleic acid targeting integrin (33, we have provided evidence for the involvement of lipid rafts, c-SRC and integrin αVβ3in regulating A375cell migration. Coimmunoprecitation and confocal immunofluorescence assays studies reveal that lipid raft disruption does not affect the interaction of c-SRC and integrin αVβ3. However, we notice that lipid raft disruption reduces the number of focal adhesions indicating that lipid rafts may probably affect integrin αVβ3clustering thus affecting A375cell migration. Furthermore, since lipid rafts did not affect the direct interaction of the c-SRC and integrin αVβ3suggests that lipid rafts and c-SRC may independently regulate integrin αVβ3to affect cell migration.The ability of these components to mediate A375cell migration provides a probable explanation for their association with a variety of cancer disorders. |
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