Adhesive Ability Of Mycobacterium Tuberculosis Onto The Surface Of Different Joint Prosthesis Material

Objective: To investigate the influence of artificial joint prosthesis with propertiesand surfaces of different materials on the adhesion abilities of Mycobacteriumtuberculosis.Methods: Specimens of titanium alloy and cobalt chromium molybdenumalloy were made respectively (with smooth surface and rough surface, n=5),Mycobacterium tuberculosis was labeled by FITC after subculture to be made intobacterial liquid; at the same time,choose Staphylococcus epidermidis for controlstudy.Bacteria liquid and samples were co-cultured at37℃for24h and then be observedusing fluorescence microscopy and scanning electron microscopy to identify adhesionconditions of two bacterium. Results: The adhesion areas of Staphylococcus epidermidisand Mycobacterium tuberculosis on cobalt chromium molybdenum alloy were larger thantitanium alloy respectively; in the case of the same material, the rough surface was moreprone to bacterial adhesion than the smooth surface; there were biofilm formation forStaphylococcus epidermidis on the surface of two kinds of materials, while there was nobiofilm formation for Mycobacterium tuberculosis.Conclusion: This study suggests thatthe property and the roughness of surface of the material have tremendous effects on theadhesion abilities of Mycobacterium tuberculosis; and there were no biofilm formation forMycobacterium tuberculosis on the surfaces of cobalt chromium molybdenum alloy andtitanium alloy.