The Role Of STIM1in The Proliferation Of Melanoma

Objective:The ubiquitous second messenger Ca2+is one of the crucial signalings that modulate various cellular functions. The dysregulation of Ca2+homeostasis has been suggested as an important event in the proliferation of malignant tumor. Stromal interaction molecule1(STIM1), the Ca2+sensor located ER, can trigger a cascade of reactions leading to SOCE activation. Aberrant expression of STIM1could be observed in several human cancer cells. However, the role of STIM1in regulating cancer progression remains controversial. The aim of current study is to confirm the role of STIM1in proliferation of melanoma, which would provide scientific basis for the further understanding of the molecular mechanisms in progression of melanoma.Methods:●Expression of STIM1in human melanoma cell lines A2058and G361was evaluated by westernblotting.●STIM1protein of G361cell was knockdown by lentvirus-mediated short harpin RNA (shRNA). Westernblotting were performed to detect the protein expression of STIM1after infection.●MTS cell proliferation assay, cell counting and flow cytometry cell cycle analysis were used to analyze the alterations of G361cell proliferation ability.●By immunochemical ABC techenique, we detected the expression of STIM1in the pathological paraffin tissue of melanoma and the begnin nevusResults:●STIM1are stable expressed in human melanoma cell lines (A2058and G361) measured by westernblotting.●Westernblotting results showed that STIM1was kcockdown by STIM1-shRNA lentiviral vector.●MTS cell proliferation assay indicated that cell proliferation were suppressed, flow cytometry cell cycle analysis results revealed cell cycle was significantly arrest at G0/G1after STIM1downregulation.●The expression of STIM1of melanoma is significantly higher, as compared with begnin nevus(66.7%vs.20.0%, p<0.05)Conclusions:●Dysregulation of STIM1plays a key role in the regulation of proliferation of melanoma cell.●Melanoma tissues have abnormally high expression of STIM1, suggesting that STIM1is related.with tumorigenesis of melanoma possibly.