The Study On Treatment Of Spinal Cord Injury By Human Umbilical Cord Mesenchymal Stem Cell And Ibuprofen

ObjectiveTo investigate the effects of ibuprofen combined with human umbilical cord mesenchymal stem cell (hUCB-SCs) transplantation in the treatment of spinal cord injury in rats, and explore the influence of ibuprofen on the survival and differentiation of hUC-MSCs.MethodsThe P5hUCB-SCs were provided by Tianjin Concord Stem Cell Geng Engineering Company. Then the morphological features were observed with inverted micro-scope and the phenotypic characterization was detected by flow cytometry.Eighty Female Wistar rats were randomly divided into four groups:DMEM control group (group A, n=20), Ibuprofen group (group B, n=20), hUC-MSCs group (group C, n=20), co-transplantation (group D, n=20). The SCI animal models were induced by Impactor Model II at T10. On days1and3and the last days of the following weeks (until the8th week) after transplantation, the BBB scale(Basso, Beattie&Bresnahan locomotor rating scale) was performed to evaluate the hindlimb functional recovery in8rats of each goup.At8weeks, all the rats were sacrificed and the spinal cords with injured lesion were taken out and made into paraffin sections (5μm). The survival of hUC-MSCs was observed with immunochemistry (MAB1281), and the differentiation of hUC-MSCs was observed with immunochemistry (NF-200).The pictures were processed by microimage analysis software (Image pro plus6.0). Positive response area was presented as mean±s.d. BBB scale and positive response area differences among groups were analyzed using the one-way analysis of variance (ANOVA), and SNK(Student-Newman-Keuls) for multiple comparisons were used to determine significant differences. All statistical tests were performed using the Software Package SPSS version16.0. The two-sided significance level was set at P<0.05.ResultsThe hUCB-SCs of P5were in regular morphology with swirling growth. The expression of CD73、CD105and Anti-HLA-ABC were positive.In addition, the expression of CD34、CD45and Anti-HLA-DR were negative.The cells were consistented with the phenotypic characterization of mesenchymal stem cell.As BBB scale showed, group D had significantly higher score than the other groups from5weeks after transplantation and the differences existed until8weeks after transplantation. At8weeks, BBB scale in group D(0.28±0.05)was higher than group B(10.57±2.31)、group C(11.91±1.88). The difference between them were statistically significant (P<0.05).Immunohistochemical staining showed the number of surviving hUC-MSCs in group D were more than other groups at8weeks.In addition, the NF-200positive area in group D(0.28±0.05)was higher than group B(0.11±0.04)、group C (0.18±0.06). The difference between them were statistically significant (P<0.05).ConclusionIbuprofen can promote the survival and differentiation of the transplanted hUC-MSCs. Co-transplantation of hUC-MSCs and ibuprofen may significantly promote axonal regeneration and functional recovery after spinal cord injury.